Recombinant functional human lactoferrin expressed in baculovirus system

Acta Biochimica Et Biophysica Sinica
Tao LiuXiang-Fu Wu

Abstract

Human lactoferrin (hLf) is a multifunctional iron-binding glycoprotein. In this study, we amplified hLf cDNA by reverse transcription-polymerase chain reaction from normal human mammary gland. The nucleotide sequence of the hLf was identical to the known hLf. We constructed a recombinant virus, vBm-hLf, harboring the hLf gene and exploited the BmN cells as host to produce recombinant human lactoferrin (rhLf). It was found that a recombinant protein with a molecular mass of approximately 78 kDa was expressed. Approximately 13.5 microg rhLf was purified from 1-2x10(5) BmN cells infected by vBm-hLf and the rhLf proved to be biologically active. This method established in our study will pave the way for efficient production of rhLf for further application of this protein in the future.

References

Sep 11, 1990·Nucleic Acids Research·M W ReyF R Pieper
Jan 1, 1974·Advances in Experimental Medicine and Biology·R M Parry, E M Brown
Aug 15, 1995·Biochemical and Biophysical Research Communications·K Kadono-OkudaK Nakashima
Mar 1, 1997·Protein Expression and Purification·V SalmonG Spik
Mar 28, 1997·The Journal of Biological Chemistry·J H NuijensF R Pieper
Feb 12, 1998·Journal of Molecular Biology·S A MooreE N Baker
May 8, 1998·Biochemical and Biophysical Research Communications·M IkedaN Kato
Jun 19, 1998·Protein Expression and Purification·V SalmonB Mérot
Sep 21, 2000·Early Human Development·Y AnderssonO Hernell
Jan 3, 2003·Protein Expression and Purification·Deepak SehgalShahid Jameel
Jul 5, 2005·Journal of Biotechnology·Tao LiuXiang-Fu Wu

❮ Previous
Next ❯

Citations

Jul 6, 2011·Biochimica Et Biophysica Acta·Isui Abril García-MontoyaQuintín Rascón-Cruz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.