Recombinant guinea pig CCL5 (RANTES) differentially modulates cytokine production in alveolar and peritoneal macrophages

Journal of Leukocyte Biology
T SkworDavid N McMurray

Abstract

The CC chemokine ligand 5 (CCL5; regulated on activation, normal T expressed and secreted) is known to recruit and activate leukocytes; however, its role in altering the responses of host cells to a subsequent encounter with a microbial pathogen has rarely been studied. Recombinant guinea pig (rgp)CCL5 was prepared, and its influence on peritoneal and alveolar macrophage activation was examined by measuring cytokine and chemokine mRNA expression in cells stimulated with rgpCCL5 alone or exposed to rgpCCL5 prior to lipopolysaccharide (LPS) stimulation. Levels of mRNA for guinea pig tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-1beta, CCL2 (monocyte chemoattractant protein-1), and CXC chemokine ligand 8 (IL-8) were analyzed by reverse transcription followed by real-time polymerase chain reaction analysis using SYBR Green. Bioactive TNF-alpha protein concentration was measured using the L929 bioassay. Both macrophage populations displayed significant enhancement of all the genes and TNF-alpha protein levels when stimulated with rgpCCL5, except for CCL2 in alveolar macrophages. When peritoneal or alveolar macrophages were pretreated with rgpCCL5 for 2 h and then exposed to low concentrations of LPS, diminished cytokine ...Continue Reading

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Citations

Jun 30, 2012·Molecular Biotechnology·Vijaya R DirisalaDavid N McMurray
Jul 3, 2013·Molecular Biotechnology·Vijaya R DirisalaDavid N McMurray
Sep 25, 2010·The Journal of Infectious Diseases·Lee G KlinkenbergPetros C Karakousis
May 5, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Hubert SchäferReinhard Burger
Nov 28, 2012·The Journal of Pain : Official Journal of the American Pain Society·Jiin-Tarng LiouYuan-Ji Day
Feb 22, 2012·Gene·Vijaya R DirisalaDavid N McMurray
May 9, 2008·Pulmonary Pharmacology & Therapeutics·Brendan J Canning, Yangling Chou
Mar 4, 2006·Microbes and Infection·JoAnne L Flynn
Oct 27, 2005·Tuberculosis·U D Gupta, V M Katoch
May 23, 2015·Mediators of Inflammation·Vijaya R DirisalaDavid N McMurray
Apr 10, 2012·Life Sciences·Shunsuke IshizakiKoichiro Tatsumi
Nov 24, 2019·Molecular Biotechnology·Madhavan OmanakuttanDavid McMurray
Oct 12, 2016·Microbiology Spectrum·Ian M Orme, Diane J Ordway

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