Recombinant human bone morphogenetic protein-9 potently induces osteogenic differentiation of human periodontal ligament fibroblasts

European Journal of Oral Sciences
Sawako FuchigamiKazuyuki Noguchi

Abstract

To accomplish effective periodontal regeneration for periodontal defects, several regenerative methods using growth and differentiation factors, including bone morphogenetic proteins (BMPs), have been developed. Bone morphogenetic protein-9 exhibits the most potent osteogenic activity of this growth factor family. However, it is unclear whether exogenous BMP-9 can induce osteogenic differentiation in human periodontal ligament (PDL) fibroblasts. Here, we examined the effects of recombinant human (rh) BMP-9 on osteoblastic differentiation in human PDL fibroblasts in vitro, compared with rhBMP-2. Recombinant human BMP-9 potently induced alkaline phosphatase (ALP) activity, mineralization, and increased expression of runt-related transcription factor-2/core binding factor alpha 1 (RUNX2/CBFA1), osterix, inhibitor of DNA binding/differentiation-1 (ID1), osteopontin, and bone sialoprotein genes, compared with rhBMP-2. The levels of rhBMP-9-induced osterix and ALP mRNA were significantly reduced in activin receptor-like kinase-1 and -2 small interfering RNA (siRNA)-transfected human PDL fibroblasts. Recombinant human BMP-9-induced ALP activity was not inhibited by noggin, in contrast to rhBMP-2 induced ALP activity, which was. Phosph...Continue Reading

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Citations

Sep 13, 2018·Journal of Cellular Physiology·Yanee TantilertanantNeeracha Sanchavanakit
Apr 3, 2019·The International Journal of Artificial Organs·Daniela CarmagnolaElena Varoni
Jul 1, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elisa MazzoniMauro Tognon
Feb 6, 2018·Journal of Biomedical Materials Research. Part a·Masako Fujioka-KobayashiRichard J Miron
Jul 2, 2019·Science and Technology of Advanced Materials·Anongwee LeewananthawetToshihiko Suzuki
Sep 9, 2019·ACS Biomaterials Science & Engineering·Bipin GaihreAmbalangodage C Jayasuriya
Aug 28, 2021·International Journal of Molecular Sciences·Hang YuanSatoru Yamada

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