Mar 1, 1990

Recombinant human bone morphogenetic protein induces bone formation

Proceedings of the National Academy of Sciences of the United States of America
E A WangP LaPan

Abstract

We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.

  • References15
  • Citations789

References

Mentioned in this Paper

Proteins, Recombinant DNA
Blastocyst Implantation, Natural
BMP2
Bone Morphogenetic Proteins
Growth Substances
Bone morphogenetic protein 2
Bmp1
Recombinants
Bone Growth
Assay

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