Recombinant human brain natriuretic peptide regulates PI3K/AKT/mTOR pathway through lncRNA EGOT to attenuate hypoxia-induced injury in H9c2 cardiomyocytes

Biochemical and Biophysical Research Communications
Chengxi ZhangYesheng Ling

Abstract

This study aimed to investigate whether recombinant human brain natriuretic peptide (rhBNP) regulated hypoxia-induced injury in H9c2 cardiomyocytes through lncRNA EGOT. H9c2 cardiomyocytes were cultured under normoxia and hypoxia (21% and 3% O2) conditions, and whether hypoxia induced injury by assessing cell viability, apoptosis and autophagy. H9c2 cells were then treated with different doses of exogenous rhBNP (200, 600 and 900 nmol/L, respectively) and the effects of rhBNP on hypoxia-induced injury in H9c2 cells as well as the expression of EGOT were studied. In addition, the regulatory relationships between rhBNP and EGOT as well as between rhBNP and PI3K/AKT/mTOR pathway in hypoxia-treated H9c2 cells were investigated. Hypoxia significantly induced injury in H9c2 cells (inhibited cell viability and promoted cell apoptosis and autophagy) and decreased the expression of EGOT. However, administration of rhBNP alleviated hypoxia-induced injury in H9c2 cells and elevated expression of EGOT. Suppression of EGOT significantly reversed the effects of rhBNP on hypoxia-induced injury in H9c2 cells. Further studies showed that the effects of EGOT on cell viability and apoptosis were by positively regulating the expression of Cyclin D...Continue Reading

Citations

Aug 26, 2020·Journal of Cardiovascular Pharmacology and Therapeutics·Andrey V KrylatovLeonid N Maslov
Jul 23, 2020·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Fenghua LvYulei Chen
Dec 19, 2020·Molecular Therapy. Nucleic Acids·Jinning GaoKai Shao
Mar 29, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Lee S ToniCarmen C Sucharov
Apr 2, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marina BarriocanalPuri Fortes

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