Recombinant human epidermal growth factor (rhEGF) protects radiation-induced intestine injury in murine system

Journal of Radiation Research
Hae Jin OhMihee Song

Abstract

This study was to investigate whether rhEGF protects radiation induced intestine injury without compromising antitumor effect of radiation in murine system. A radiation induced intestinal injury model was established in mice by whole body irradiation. Using this model, 4 groups were set; control, rhEGF (100 µg/kg intraperitoneally), radiation (10 Gy), and a combination (rhEGF and radiation). The level of apoptosis and proliferation were analyzed by TUNEL assay and proliferation cell nuclear antigen (PCNA) immunohistochemical staining, respectively, as well as observation of survival and body weight change. A tumor growth delay assay was performed using murine syngeneic tumors; one radioresistant tumor, HCa-I and one radiosensitive tumor, MCa-K. In the radiation induced intestinal injury model, the 10 Gy group had significantly more weight loss with less number of crypt cells and higher apoptosis than the 8 Gy group. Using 10 Gy model, radioprotective effect of rhEGF was tested. Addition of rhEGF improved not only the body weight loss but also survival following radiation. It also induced suppression of apoptosis as well as increase of PCNA expression and recovery of villi. rhEGF did not enhance the tumor growth after radiation ...Continue Reading

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Citations

Jan 10, 2012·Current Opinion in Supportive and Palliative Care·Maaike Berbée, Martin Hauer-Jensen
May 23, 2015·International Journal of Radiation Biology·Jaroslav PejchalJiřina Vávrová
Jul 23, 2014·Plant Cell Reports·David Rhys Thomas, Amanda Maree Walmsley
Jul 1, 2016·Physiological Reviews·Jianchun ChenRaymond C Harris
Jun 6, 2017·Bone Marrow Transplantation·S G HoltanA Panoskaltsis-Mortari
Jun 29, 2021·Free Radical Research·Rahul CheckerSantosh K Sandur

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