Recombinant human erythropoietin reduces epithelial cell apoptosis and attenuates bleomycin-induced pneumonitis in mice

Respirology : Official Journal of the Asian Pacific Society of Respirology
M YoshimiYoichi Nakanishi

Abstract

Erythropoietin (EPO) has recently been demonstrated to have a tissue protective role by acting as an anti-apoptotic agent in various tissues, such as brain, spinal cord, heart and kidney. The purpose of this study was to determine whether human recombinant EPO reduces epithelial cell apoptosis and attenuates bleomycin-induced pneumonitis in mice. Bleomycin was instilled intratracheally into C57BL/6 mice. Recombinant human EPO or saline was injected intraperitoneally, daily from day 5 to day 13 after bleomycin instillation. EPO receptor was expressed in bronchiolar and alveolar type II cells. At 14 days after instillation, the number of terminal uridine deoxynucleotidyl transferase nick end-labelled positive cells in the lung was decreased, and the histological degree of inflammation and fibrosis was attenuated in mice injected with EPO compared with controls. Expression of phosphorylated Akt and Erk, which are thought to mediate the survival signalling pathway induced by EPO, tended to be increased in lung tissues from mice treated with EPO compared with those from mice treated with saline after bleomycin instillation. As it is likely that EPO protects epithelial cells from injury and apoptosis, EPO administration could be a po...Continue Reading

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Citations

Nov 12, 2010·European Journal of Clinical Pharmacology·Sotirios KakavasTheodoros Xanthos
Apr 9, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·Martin R J Kolb, Ian Yang
May 31, 2011·Allergologia et immunopathologia·M KaramanO Karaman
Jul 30, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mauro HeitrichMaría Victoria Aguirre
Nov 24, 2016·Pediatric Pulmonology·Hajime TakayasuKouji Masumoto
Apr 9, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Liasmine HaineNicolas Voituron
Mar 9, 2020·Revue des maladies respiratoires·C H YegenN Voituron

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis