Recombinant human interleukin-24 suppresses gastric carcinoma cell growth in vitro and in vivo

Cancer Investigation
Su YanJicheng Yang

Abstract

Previous studies have demonstrated that interleukin-24 [IL-24; originally called melanoma differentiation associated gene-7 (mda-7)] as a novel tumor suppressor gene has tumor-suppressive activity against a broad spectrum of human cancers. However, the therapeutic effect of the recombinant human IL-24 (rhIL-24) protein purified from prokaryotic cells on gastric cancer has not been reported. In this study, we purified soluble rhIL-24 using Q-Sepharose column after the denaturing and renaturing process from the protein of Escherichia coli BL21 transfected with pET-21a(+)-hIL-24 vector and treated by isopropyl-beta-D-1-thiogalactopyranoside (IPTG) for enhanced expression of transgene rhIL-24. We demonstrated that rhIL-24 was capable of inducing in vitro apoptosis of SGC7901 gastric cancer cells and activating peripheral blood mononuclear cellsto secrete cytokines such as IL-6, TNF-alpha, and IFN-gamma. We also showed that rhIL-24 was able to inhibit formation of blood capillaries on chicken embryonic allantois and in vivo tumor angiogenesis leading to suppressing SGC7901 gastric cancer cell growth in vitro and in vivo possibly due to its downregulation of Bcl-2/Bax ratio, VEGF (vascular endothelial growth factor), and CD34. Theref...Continue Reading

References

May 1, 1995·Nuclear Medicine and Biology·T HuangS Mong
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z Z SuP B Fisher
Jul 31, 2001·International Journal of Cancer. Journal International Du Cancer·Z ChenJ Xiang
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva G CaudellElizabeth A Grimm
Jul 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Devanand SarkarPaul B Fisher
Nov 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Harold F Dvorak
Dec 18, 2002·Cytokine & Growth Factor Reviews·Moira SauanePaul B Fisher
Aug 9, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·Abner M MhashilkarSunil Chada
Feb 21, 2004·CA: a Cancer Journal for Clinicians·Ahmedin JemalUNKNOWN American Cancer Society
Mar 10, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kerry A SiegerSunil Chada
May 4, 2004·International Immunopharmacology·Marehalli L NagalakshmiRene de Waal Malefyt
May 4, 2004·International Immunopharmacology·Sunil ChadaAbner M Mhashilkar
Aug 17, 2004·Immunity·Kerstin WolkRobert Sabat
Mar 25, 2005·Nature Reviews. Drug Discovery·Dong Wang, Stephen J Lippard
Feb 27, 2008·Cancer Biology & Therapy·Lili WeiFangzhou Song

❮ Previous
Next ❯

Citations

Apr 24, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Baobiao ZhuoYingchun Shi
May 23, 2013·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Yuan LiTao Han
Sep 18, 2012·Cytokine & Growth Factor Reviews·Erin L WhitakerPenelope J Duerksen-Hughes
Feb 9, 2010·Trends in Molecular Medicine·Stefan Grimm, Mathieu Noteborn
Aug 3, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lijie YuanXinghan Liu
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hongdan CaoXiaolan Huang
Jun 28, 2017·Cancer Chemotherapy and Pharmacology·Samira ValiyariSaeid Bouzari

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.