PMID: 9162521Apr 1, 1996Paper

Recombinant Interferons beta and gamma have a higher antiviral activity than interferon-alpha in coxsackievirus B3-infected carrier state cultures of human myocardial fibroblasts

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
A Heim Figulla H-R

Abstract

We compared the antiviral activities of three recombinant human interferons (IFN-alph2a, IFN-beta, and IFN-gamma) in cultured human myocardial fibroblasts to select a candidate for trial in heart disease induced by cardiotropic enterovirus, e.g., coxsackievirus B3 (CVB3). Cells were exposed to CVB3, and after 7 days, when a persistent infection had developed, IFN was added. Virus yields were measured on alternate days for the next 7 or 16 days, and IFN activity was assessed as the percentage reduction in yield. IFN-gamma and IFN-beta were both highly active and reduced virus yields by 2 log (EC(99)) at concentrations of 23.4 IU/ml (SD = 8.6) and 10.1 IU/ml (SD = 3.2), respectively; with 250 IU/ml of either IFN, no infectious virus was formed. Unexpectedly, IFN-alpha2a (EC(99)> 1250 IU/ml) was at least 120 times less active than IFN-beta; after use for 8 days or more, the minor effects it produced were no longer related to the concentration applied. Despite the pharmacokinetic advantages of IFN-alpha2a, our data suggest that IFN-beta should in preference be evaluated in the clinic.

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Citations

Jun 5, 2012·Medical Microbiology and Immunology·Fabienne RehrenAlbert Heim
Feb 5, 2002·Veterinary Immunology and Immunopathology·Falko SteinbachIlka Beier
Jul 19, 2000·Current Opinion in Immunology·C Bogdan
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·C ZaragozaC J Lowenstein
Jun 14, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Jonathan LarkinJohn I Glass
Aug 24, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·I M GrumbachL C Platanias
Oct 29, 2005·Journal of Clinical Pathology·J K S Chia
Dec 5, 2008·Expert Review of Vaccines·Andreas HenkePeter Wutzler
Jan 20, 2017·Journal of Virology·Christopher M RobinsonJulie K Pfeiffer
Jul 14, 2004·Circulation·Raj DeonarainEleanor N Fish
Aug 8, 2013·Journal of the American Heart Association·Julian K S KimIraklis I Pipinos
Jan 15, 2008·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Luigi Brunetti, Evelyn R Hermes DeSantis
Apr 4, 2017·Archives of Toxicology·Giorgia PalloccaMarcel Leist

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