Recombinant interleukin 6 with M cell-targeting moiety produced in Lactococcus lactis IL1403 as a potent mucosal adjuvant for peroral immunization

Vaccine
Hui-Shan LiYun-Jaie Choi

Abstract

Development and application of safe and effective mucosal adjuvants are important to improve immunization efficiency in oral vaccine. Here, we report a novel mucosal adjuvant, IL-6-CKS9, a recombinant cytokine generated by conjugating an M cell-targeting peptide (CKS9) with c-terminus of the murine interleukin 6 (IL-6), which facilitated enhancement of mucosal immune response. Lactococcus lactis IL1403, a food-grade strain of lactic acid bacteria (LAB) which is widely used in dairy industry, was used as a host cell to express and secrete the IL-6-CKS9 for a mucosal vaccine adjuvant. The recombinant L. lactis IL1403 secreting IL-6-CKS9 was orally administered with a model antigen protein, M-BmpB (Brachyspira membrane protein B conjugated with CKS9), to BALB/c mice for mucosal immunization. ELISA analyses showed consistent enhancement tendencies in induction of anti-M-BmpB antibody levels both with mucosal (IgA) and systemic (IgG) immune responses in IL-6-CKS9-LAB treated group compared with other groups tested by conducting two separated mice immunization assays. In addition, we characterized that the oral administration of model protein antigen with live LAB producing IL-6-CKS9 could induce both Th1 and Th2 type immune response...Continue Reading

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Citations

May 8, 2016·Applied Microbiology and Biotechnology·Miao WangLi Pan
Apr 6, 2017·Microbial Cell Factories·Adelene Ai-Lian SongRaha Abdul Rahim
Jan 19, 2019·Applied Microbiology and Biotechnology·Tina Vida Plavec, Aleš Berlec
Jun 9, 2019·Applied Microbiology and Biotechnology·Keita TakahashiNaoki Inoue
Dec 2, 2017·Biotechnology & Genetic Engineering Reviews·Daniel Bravo, José M Landete
Jul 10, 2019·Frontiers in Microbiology·Janette V PhamYeo Joon Yoon
Nov 16, 2018·Biomaterials·Mohammad Ariful IslamChong Su Cho
Mar 20, 2021·Essays in Biochemistry·Michael Sauer, Nam Soo Han
Jun 21, 2021·Microbial Cell Factories·Jiapeng WuTingting Guo

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