Recombinant Lactobacillus plantarum expressing HA2 antigen elicits protective immunity against H9N2 avian influenza virus in chickens

Applied Microbiology and Biotechnology
Wen-Tao YangChun-Feng Wang

Abstract

Low pathogenic H9N2 subtype avian influenza virus (AIV) can lead to moderate respiratory symptoms and low egg production rates in poultry. Due to its immunologic suppression, other various infectious pathogens give rise to the co-infection of hosts, causing heavy economic losses in the commercial poultry industry in both China and worldwide. Therefore, it is time to explore a novel, safe, and effective vaccine. We have already made use of the surface of Lactobacillus plantarum to display AIV HA2 (NC8-pSIP409-pgsA'-HA2), which demonstrated that it has a good immunogenicity. In this study, by evaluating the immune protection effect of NC8-pSIP409-pgsA'-HA2 on chickens, we found that the hemagglutination inhibition (HI) antibodies, specificity IgG antibody in chickens, the sIgA titer in broncho alveolar lavage fluids (BALF), and the T cell response were increased notably after oral vaccination with NC8-pSIP409-pgsA'-HA2. In addition, weight loss, lung titers, and lung pathologies were improved when chickens were orally vaccinated with NC8-pSIP409-pgsA'-HA2 after challenge with H9N2 AIV. This strategy lays the foundation for the development of recombinant L. plantarum oral vaccines in the prevention of AIV.

References

Apr 29, 2006·International Journal of Cancer. Journal International Du Cancer·Haryoung PooSeung-Hoon Lee
Jul 9, 2011·Science·Damian C EkiertJaap Goudsmit
Jul 5, 2013·Nature·Jianfang ZhouYuelong Shu
Apr 29, 2014·Applied Microbiology and Biotechnology·Chengcheng RenWei Chen
Apr 14, 2015·Applied Microbiology and Biotechnology·Agnieszka WyszyńskaElżbieta Katarzyna Jagusztyn-Krynicka
Jul 30, 2015·The New England Journal of Medicine·Zi-Feng YangNan-Shan Zhong
Mar 30, 2016·Applied Microbiology and Biotechnology·Graziano CaggianielloGiuseppe Spano
Apr 3, 2016·Applied Microbiology and Biotechnology·Panagiotis Chanos, Tina Mygind

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Citations

Jun 20, 2020·Journal of Animal Physiology and Animal Nutrition·Ligang XueWeimin Luan
Dec 31, 2020·Vaccines·Hans Van der WekenBert Devriendt
May 9, 2021·Acta Biochimica Et Biophysica Sinica·Seria Masole ShonyelaChunfeng Wang

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Datasets Mentioned

BETA
KM245331
MF620131

Methods Mentioned

BETA
fluorescence
flow cytometry
flow
fluorescence-activated
FCS
ELISA

Software Mentioned

GraphPad Prism
DNAStar MegAlign
FlowJo

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