Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis

Veterinary Microbiology
Sohini DeyK Nachimuthu

Abstract

A recombinant antigen-based single serum dilution enzyme-linked immunosorbent assay (ELISA) was developed to measure the specific antibody activity in sera of dogs with leptospirosis. The recombinant antigen developed and used in the assay was specific for the pathogenic serovars of Leptospira. A linear relationship was found to exist between the predicted antibody titres at a single working dilution of 1:1000 and the corresponding observed serum titres as determined by the standard serial-dilution method. Regression analysis was used to determine a standard curve from which an equation can be derived that allows demonstration of the mentioned correlation. The equation was then used to convert the corrected absorbance readings of the single working dilution directly into the predicted ELISA antibody titres. The assay was proved to be sensitive, specific and accurate as compared to the standard microscopic agglutination test (MAT).

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Citations

Apr 11, 2014·Iranian Red Crescent Medical Journal·Ebrahim Khodaverdi DarianPejvak Khaki
May 16, 2014·Iranian Red Crescent Medical Journal·Safar Ali AlizadehReza Najafipour
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Aug 19, 2006·Canadian Journal of Microbiology·Sandra Denize Dorneles JouglardOdir Antônio Dellagostin
Sep 22, 2009·Canadian Journal of Microbiology·Wei ZhaoXiao-Kui Guo
Dec 23, 2006·Veterinary Research Communications·S DeyK Nachimuthu
Jan 16, 2007·Veterinary Research Communications·Sohini DeyK Nachimuthu
May 30, 2009·Journal of Molecular Biology·Pricila HaukChuck Shaker Farah
Mar 25, 2009·Comparative Immunology, Microbiology and Infectious Diseases·F AviatG Andre-Fontaine
Jun 21, 2007·Microbiology and Immunology·Michiko NaitoHiroshi Kida
May 22, 2013·Critical Reviews in Microbiology·Veerapandian Raja, Kalimuthusamy Natarajaseenivasan
Feb 11, 2015·Comparative Immunology, Microbiology and Infectious Diseases·Geneviève Andre-FontaineBenoit Chatrenet
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Jun 14, 2005·Veterinary Microbiology·Maria Rosa Quaresma BomfimMatilde Cota Koury
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Mar 1, 2013·Expert Review of Clinical Immunology·Stefan SchreierWannapong Triampo
Sep 19, 2018·BMC Veterinary Research·Liping YanJiyong Zhou

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