Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera

Veterinary Microbiology
Ludwig E HoelzleM M Wittenbrink

Abstract

Recombinant major outer membrane proteins (rMOMP) of Chlamydophila (Ch.) abortus, Ch. pecorum, and Chlamydia (C.) suis were used as antigens to distinguish chlamydial species-specific antibodies in (i) immune sera from six rabbits and three pigs raised against native purified elementary bodies, (ii) serum samples from 25 sows vaccinated with Ch. abortus, and (iii) 40 serum samples from four heifers experimentally infected with Ch. abortus. All post-exposition sera contained chlamydial antibodies as confirmed by strong ELISA seroreactivities against the chlamydial LPS. For the rMOMP ELISA mean IgG antibody levels were at least 5.8-fold higher with the particular rMOMP homologous to the chlamydial species used for immunisation or infection than with heterologous rMOMPs (P <0.001). Preferential rMOMP ELISA reactivities of sera were confirmed by Western blotting. The results suggest that the entire chlamydial rMOMP could provide a species-specific serodiagnostic antigen.

References

Aug 1, 1993·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M M WittenbrinkW Bisping
Jul 13, 2000·Veterinary Microbiology·K D Everett
Nov 25, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·L E HoelzleM M Wittenbrink

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Citations

Feb 15, 2011·Veterinary Research·Katelijn Schautteet, Daisy Vanrompay
Nov 7, 2008·Veterinary Microbiology·Konrad SachseDavid Longbottom
Jul 10, 2009·Clinical and Vaccine Immunology : CVI·Irene SchillerBruno Oesch
Feb 10, 2009·The Veterinary Record·M DonatiR Cevenini

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