Recombinant measles vaccine expressing malaria antigens induces long-term memory and protection in mice

NPJ Vaccines
Marie MuraFrédéric Tangy

Abstract

Following the RTS,S malaria vaccine, which showed only partial protection with short-term memory, there is strong support to develop second-generation malaria vaccines that yield higher efficacy with longer duration. The use of replicating viral vectors to deliver subunit vaccines is of great interest due to their capacity to induce efficient cellular immune responses and long-term memory. The measles vaccine virus offers an efficient and safe live viral vector that could easily be implemented in the field. Here, we produced recombinant measles viruses (rMV) expressing malaria "gold standard" circumsporozoïte antigen (CS) of Plasmodium berghei (Pb) and Plasmodium falciparum (Pf) to test proof of concept of this delivery strategy. Immunization with rMV expressing PbCS or PfCS induced high antibody responses in mice that did not decrease for at least 22 weeks post-prime, as well as rapid development of cellular immune responses. The observed long-term memory response is key for development of second-generation malaria vaccines. Sterile protection was achieved in 33% of immunized mice, as usually observed with the CS antigen, and all other immunized animals were clinically protected from severe and lethal Pb ANKA-induced cerebral ...Continue Reading

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Citations

Feb 27, 2020·Viruses·Elena BuschChristine E Engeland
Apr 8, 2020·Frontiers in Public Health·Hongli GuanXiaohong Peng
May 4, 2021·Frontiers in Molecular Biosciences·Rajashri BezbaruahSaeid Abu-Romman
Dec 15, 2020·ACS Biomaterials Science & Engineering·Lampouguin Yenkoidiok-Douti, Christopher M Jewell

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Methods Mentioned

BETA
flow cytometry
FCS
FACS

Software Mentioned

Flow Jo TM

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