Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin β3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury

Cell Death & Disease
Yongyue GaoChun-Hua Hang

Abstract

Accumulating evidence suggests neuronal apoptosis has the potential to lead to more harmful effects in the pathological processes following traumatic brain injury (TBI). Previous studies have established that milk fat globule-EGF factor-8 (MFG-E8) provides neuroprotection through modulation of inflammation, oxidative stress, and especially apoptosis in cerebral ischemia and neurodegenerative disease. However, the effects of MFG-E8 on neuronal apoptosis in TBI have not yet been investigated. Therefore, we explored the role of MFG-E8 on anti-apoptosis and its potential mechanism following TBI. In the first set of experiments, adult male Sprague-Dawley (SD) rats were randomly divided into Sham and TBI groups that were each further divided into five groups representing different time points (6 h, 24 h, 72 h, and 7 days) (n = 9 each). Western blotting, quantitative real-time PCR, and immunofluorescence staining were performed to identify the expression and cellular localization of MFG-E8. In the second set of experiments, four groups were randomly assigned: Sham group, TBI + Vehicle group, and TBI + rhMFG-E8 (1 and 3 µg) (n = 15). Recombinant human MFGE8 (rhMFG-E8) was administrated as two concentrations through intracerebroventricu...Continue Reading

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Citations

Dec 7, 2019·Stem Cell Reviews and Reports·Jiaying YuanXuehong Liu
Jul 10, 2019·Experimental and Therapeutic Medicine·Man ZhangDa-Qing Chen
Jun 20, 2019·Frontiers in Neuroscience·Cletus CheyuoPing Wang
Sep 18, 2021·Acta Neuropathologica Communications·Paula MarazuelaMar Hernández-Guillamon
Nov 2, 2021·Frontiers in Physiology·Yan XiongYaqiu Lin

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Methods Mentioned

BETA
Protein Assay
electrophoresis
X-ray
PCR
gene knock-out

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