Recombinant myelin-associated glycoprotein confers neural adhesion and neurite outgrowth function

Neuron
P W JohnsonJ C Roder

Abstract

Myelin-associated glycoprotein (MAG) cDNA clones for the small (p67) and large (p72) forms were expressed in heterologous cells. Purified recombinant MAG protein was incorporated into fluorescent liposomes, and both forms were shown to bind predominantly to neurites in DRG or spinal cord cultures. This adhesion was completely blocked by Fab fragments of monoclonal anti-MAG antibody. Liposomes prepared with the control protein glycophorin or no protein failed to bind neurites. Small cerebellar neurons, which are not myelinated in vivo, failed to bind MAG liposomes. In a second test of function, p67 MAG-transfected fibroblasts were markedly enhanced in their ability to promote DRG neurite extension over a 2 day culture period compared with control fibroblasts not expressing MAG. Neurite extension was blocked by anti-MAG antibodies. These results show that both forms of MAG can facilitate the interactions between glial cells and neurites that ultimately lead to myelin formation.

References

Dec 1, 1976·The Journal of General Virology·N D Stow, N M Wilkie
Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N H SternbergerH D Webster
Feb 1, 1986·The Journal of Cell Biology·M Dubois-DalcqR A Lazzarini
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M ArquintR Dunn
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B Seed, A Aruffo
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C LaiJ G Sutcliffe
Sep 1, 1988·Brain Research·M B TropakJ C Roder
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G M EdelmanW J Gallin
Jan 1, 1983·Developmental Neuroscience·R H Quarles
Dec 1, 1981·Journal of Neuroimmunology·J Schnitzer, M Schachner
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jan 1, 1987·Immunology Today·A F Williams

❮ Previous
Next ❯

Citations

Jul 22, 1998·Microscopy Research and Technique·S Raval-Fernandes, L H Rome
Jun 1, 1996·Journal of Neuroscience Research·B Q KreiderD Pleasure
Apr 29, 1999·Journal of Neuroscience Research·H YangM Schachner
Nov 1, 1990·Journal of Neuroscience Research·J Schneider-SchauliesM Schachner
Feb 1, 1991·Journal of Neuroscience Research·J M Le BeauG Walter
Jun 1, 1991·Journal of Neuroscience Research·L PedrazaJ L Salzer
Aug 1, 1991·Journal of Neuroscience Research·D E AfarJ C Bell
Jun 15, 1995·Journal of Neuroscience Research·A Meyer-FrankeM Schachner
Oct 1, 1995·Journal of Neurocytology·B Q KreiderD Pleasure
Feb 1, 1997·Journal of Molecular Neuroscience : MN·R H Quarles
Apr 1, 1995·Trends in Neurosciences·M Schachner, R Martini
Aug 1, 1990·Brain Research. Molecular Brain Research·N StahlB Popko
Oct 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M B TropakJ C Roder
Oct 1, 1992·Current Opinion in Neurobiology·P Doherty, F S Walsh
Nov 25, 2003·Current Opinion in Neurobiology·Glenn Yiu, Zhigang He
Aug 26, 2003·Brain Research. Developmental Brain Research·Patrick Vourc'hChristian Andres
May 25, 1999·Progress in Neurobiology·R Probstmeier, P Pesheva
Apr 30, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A M Turnley, P F Bartlett
Jan 1, 1996·Nature Medicine·J Nash, A Pini
Jul 22, 2006·Nature Reviews. Neuroscience·Glenn Yiu, Zhigang He
Jan 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·L J YangR L Schnaar
Apr 1, 1990·The Journal of Cell Biology·J L Bixby, R Zhang
May 1, 1992·The Journal of Cell Biology·P DurbecG Rougon
Nov 1, 1994·The Journal of Cell Biology·H E BeggsP F Maness
Dec 1, 1995·The Journal of Cell Biology·L BöB D Trapp
Jun 4, 2010·Cold Spring Harbor Perspectives in Biology·Roman J GigerMark H Tuszynski
Sep 1, 1993·The European Journal of Neuroscience·T FahrigM Schachner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in AS

Cell adhesion molecules expressed on the vascular endothelium and circulating leukocytes in response to inflammatory stimuli are implicated in atherosclerosis. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.