Recombinant N-terminal outer membrane porin (OprF) of Pseudomonas aeruginosa is a promising vaccine candidate against both P. aeruginosa and some strains of Acinetobacter baumannii

International Journal of Medical Microbiology : IJMM
Mohammed Bahey-El-DinTaha Ibrahim Zaghloul

Abstract

Pseudomonas aeruginosa is an evolving pathogen which can cause serious infections especially to immunocompromised patients. Its high resistance profile to antibiotics results in difficulty, and sometimes impossibility, in treating afflicted patients. Developing an effective vaccine against P. aeruginosa is an important approach to tackle this problem. A similar problematic situation exists for Acinetobacter baumannii. Several vaccine candidates have been investigated up till now but still there is no approved vaccine in the market. One important antigen of P. aeruginosa is the outer membrane protein F (OprF) which functions as a porin with relevant important roles in virulence. Previous studies focused mainly on the C-terminal peptidoglycan binding domain of OprF as a vaccine candidate. In the current study, we have investigated the N-terminal porin domain of OprF as a potential vaccine candidate against P. aeruginosa. Histidine-tagged recombinant N-terminal OprF (amino acid range 25-200; OprF25-200) was overexpressed in Escherichia coli and purified using metal affinity chromatography. Swiss albino mice were immunized with OprF25-200 adjuvanted with Bacillus Calmette-Guérin (BCG) and alum and the immune response was evaluated....Continue Reading

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Citations

Dec 9, 2020·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
Feb 9, 2021·Expert Review of Vaccines·Crystal Ma, Wangxue Chen
Nov 18, 2020·International Journal of Molecular Sciences·Corneliu Ovidiu VrancianuMariana Carmen Chifiriuc
May 7, 2021·FEMS Microbiology Reviews·Mireia López-SilesMichael J McConnell
May 12, 2021·Life Science Alliance·Géraldine MayeuxJean-Luc Lenormand

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