Recombinant NAD-dependent SIR-2 protein of Leishmania donovani: immunobiochemical characterization as a potential vaccine against visceral leishmaniasis

PLoS Neglected Tropical Diseases
Rajendra K BahariaA Dube

Abstract

The development of a vaccine conferring long-lasting immunity remains a challenge against visceral leishmaniasis (VL). Immunoproteomic characterization of Leishmania donovani proteins led to the identification of a novel protein NAD+-dependent Silent Information regulatory-2 (SIR2 family or sirtuin) protein (LdSir2RP) as one of the potent immunostimulatory proteins. Proteins of the SIR2 family are characterized by a conserved catalytic domain that exerts unique NAD-dependent deacetylase activity. In the present study, an immunobiochemical characterization of LdSir2RP and further evaluation of its immunogenicity and prophylactic potential was done to assess for its possible involvement as a vaccine candidate against leishmaniasis. LdSir2RP was successfully cloned, expressed and purified. The gene was present as a monomeric protein of ~45 kDa and further established by the crosslinking experiment. rLdSir2RP shown cytosolic localization in L. donovani and demonstrating NAD+-dependent deacetylase activity. Bioinformatic analysis also confirmed that LdSir2RP protein has NAD binding domain. The rLdSir2RP was further assessed for its cellular response by lymphoproliferative assay and cytokine ELISA in cured Leishmania patients and ham...Continue Reading

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Citations

Nov 26, 2015·Acta Tropica·Vívian Tamietti MartinsEduardo Antonio Ferraz Coelho
Oct 30, 2016·Parasite Immunology·M BagirovaB Ergenoglu
May 18, 2020·Parasite Immunology·Satish Chandra PandeyMukesh Samant
May 14, 2016·Parasites & Vectors·Saumya SrivastavaSarman Singh
Apr 11, 2019·Frontiers in Immunology·Farhat AfrinHassan A Hemeg

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Methods Mentioned

BETA
PCR
ELISA
fluorescence microscopy
cross-linking studies
density gradient centrifugation
biopsy
Fluorescence
Cross linking

Software Mentioned

Beacon Designer
CombLib Consensus
SMART
Clustal W2
NetMHCpan
CDART
GLAM2
MEME
IEDB
MEGA5

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