Recombinant oncolytic Newcastle disease virus displays antitumor activities in anaplastic thyroid cancer cells

BMC Cancer
Ke JiangSongshu Meng

Abstract

Anaplastic thyroid cancer (ATC) is one of the most aggressive of all solid tumors for which no effective therapies are currently available. Oncolytic Newcastle disease virus (NDV) has shown the potential to induce oncolytic cell death in a variety of cancer cells of diverse origins. However, whether oncolytic NDV displays antitumor effects in ATC remains to be investigated. We have previously shown that the oncolytic NDV strain FMW (NDV/FMW) induces oncolytic cell death in several cancer types. In the present study, we investigated the oncolytic effects of NDV/FMW in ATC. In this study, a recombinant NDV expressing green fluorescent protein (GFP) was generated using an NDV reverse genetics system. The resulting virus was named after rFMW/GFP and the GFP expression in infected cells was demonstrated by direct fluorescence and immunoblotting. Viral replication was evaluated by end-point dilution assay in DF-1 cell lines. Oncolytic effects were examined by biochemical and morphological experiments in cultural ATC cells and in mouse models. rFMW/GFP replicated robustly in ATC cells as did its parent virus (NDV/FMW) while the expression of GFP protein was detected in lungs and spleen of mice intravenously injected with rFMW/GFP. We ...Continue Reading

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Citations

Feb 27, 2020·Journal of Cellular and Molecular Medicine·Xueke WangQing Xu
Mar 14, 2020·Biotechnology Letters·You-Ni ZhangXiao-Zhou Mou

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Datasets Mentioned

BETA
GU564399

Methods Mentioned

BETA
xenograft
fluorescence microscopy
PCR
confocal microscopy

Software Mentioned

SPSS

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