Recombinant ovine atadenovirus induces a strong and sustained T cell response against the hepatitis C virus NS3 antigen in mice

Vaccine
Thomas WüestP Löser

Abstract

Ovine atadenovirus (OAdV) is a novel gene transfer vector with excellent in vivo gene transfer characteristics. In the present study, we have investigated the ability of an OAdV vector to mediate a T cell response to an antigen of the hepatitis C virus (HCV) in mice. Specifically, an expression cassette coding for non-structural protein 3 (NS3) of hepatitis C virus was inserted into the OAdV genome and the resulting recombinant virus (OAdV-ns3) was shown to propagate stably and to express the ns3 gene at a high level in vitro. A single injection of this non-replicating vector into BALB/c mice resulted in a strong induction of NS3-specific, IFN-gamma secreting T-lymphocytes as measured by direct ex vivo ELISpot assay. The number of IFN-gamma secreting lymphocytes remained nearly unaltered for a period of at least 10 weeks. The immune response was shown to depend on virus dose but a single intramuscular injection of less than 10(8) infectious particles of OAdV-ns3 was sufficient to induce a significant NS3-specific T cell response. Moreover, this response was not affected by prior immunisation of animals with human adenovirus type 5 (HAdV-5). The results of our study provide proof for the concept that OAdV vectors may be valuable...Continue Reading

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Citations

May 15, 2012·Expert Opinion on Biological Therapy·Luigi Aurisicchio, Gennaro Ciliberto
Jan 17, 2007·Expert Opinion on Biological Therapy·Gloria Gonzalez-AseguinolazaAstrid Paneda
Aug 4, 2009·Expert Opinion on Biological Therapy·Sridhar DharmapuriLuigi Aurisicchio
May 9, 2013·Expert Opinion on Biological Therapy·Leo SwadlingEleanor Barnes
Jul 31, 2012·Antiviral Research·Peng Peng IpToos Daemen
Nov 22, 2005·Vaccine·Dinesh S Bangari, Suresh K Mittal
Dec 9, 2014·Human Vaccines & Immunotherapeutics·Hugues Fausther-Bovendo, Gary P Kobinger
Aug 19, 2005·Nature·Michael Houghton, Sergio Abrignani
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Jan 1, 2011·Cancers·Luigi Aurisicchio, Gennaro Ciliberto
Mar 30, 2010·Expert Opinion on Therapeutic Patents·Luigi Aurisicchio, Gennaro Ciliberto
Oct 10, 2020·ACS Nano·Young Hun ChungNicole F Steinmetz

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