Abstract
1. P-selectin is involved, with P-selectin glycoprotein (GP)-ligand-1 (PSGL-1), in platelet/leukocyte interactions during thrombo-inflammatory reactions; it also stabilizes platelet aggregates. Its antagonism accelerates thrombolysis and enhances the anti-aggregatory effects of GPIIb-IIIa inhibitors. This study was designed to investigate the mechanisms of P-selectin-mediated platelet aggregation. 2. In freshly isolated human platelets, P-selectin translocation after thrombin stimulation increased rapidly to 48, 72, and 86% positive platelets after 60, 120, and 300 s, respectively. Platelet aggregation at 60 s post-stimulation averaged 46.7 +/- 1.9% and its extent followed closely the kinetics of P-selectin translocation. 3. Pre-treatment of platelets with P-selectin antagonists, a recombinant PSGL-1 (rPSGL-Ig) or a blocking monoclonal antibody, significantly delayed platelet aggregation in a dose-dependent manner. At 100 microg ml(-1) of rPSGL-Ig, platelet aggregation was completely inhibited up to 60 s post-stimulation and increased thereafter to reach maximal aggregation at 5 min. The second phase of platelet aggregation, in the presence of rPSGL-Ig, was completely prevented by the addition of a GPIIb-IIIa antagonist (Reopro...Continue Reading
References
Jun 16, 1978·Science·T K GartnerD R Phillips
Oct 1, 1994·Circulation·J E TchengE J Topol
Dec 1, 1994·Journal of the American College of Cardiology·Y JangE J Topol
Jan 1, 1994·Thrombosis Research·G B Nash
Mar 15, 1996·Journal of the American College of Cardiology·F J NeumannA Schömig
Aug 1, 1996·Journal of the American College of Cardiology·J K MickelsonC W Smith
Sep 15, 1996·Circulation·I OttA Schömig
May 1, 1997·Journal of the American College of Cardiology·C V SerranoP L da Luz
Aug 1, 1997·The Journal of Clinical Investigation·R P McEver, R D Cummings
Mar 17, 1999·Circulation·A KumarR G Schaub
Sep 28, 1999·The Journal of Experimental Medicine·G M RomoJ A López
Apr 19, 2000·The Journal of Experimental Medicine·P S FrenetteD D Wagner
Oct 18, 2000·Circulation·M Merten, P Thiagarajan
Oct 25, 2000·Circulation·M MertenP Thiagarajan
Jan 3, 2001·Anesthesiology·N FaradayT Dorman
Feb 27, 2001·Circulation·J G BienvenuY Merhi
Apr 3, 2001·Circulation·UNKNOWN Second SYMPHONY Investigators
Aug 14, 2001·Journal of the American College of Cardiology·K WangA M Lincoff
Dec 12, 2001·Circulation·M Merten, P Thiagarajan
Jun 11, 2002·Journal of Interventional Cardiology·Jonathan D Marmur, Erdal Cavusoglu
Jul 20, 2002·Journal of Cardiovascular Pharmacology·Alexandre CaronYahye Merhi
Jun 4, 2004·Thrombosis and Haemostasis·Jean-François TanguayYahye Merhi
Nov 6, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Michael MertenPerumal Thiagarajan
Citations
Jan 21, 2011·Journal of the Royal Society, Interface·Julien FatissonMaryam Tabrizian
Nov 18, 2009·Circulation·Haissam Abou-SalehYahye Merhi
Dec 16, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·C ZhangJ W Kupiec-Weglinski
Jun 10, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Letícia Gonçalves FreitasLuci Maria SantAna Dusse
Aug 20, 2009·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Eman MosadAzza A Eltayeb
Jul 15, 2009·Immunological Reviews·Douglas A CarlowHermann J Ziltener
Jul 9, 2010·Expert Opinion on Therapeutic Targets·Thilo HackertJens Werner
Jun 3, 2008·British Journal of Haematology·Simon F De MeyerHans Deckmyn
May 24, 2011·Thrombosis Research·Jean-François ThéorêtYahye Merhi