PMID: 7538327Jan 1, 1994Paper

Recombinant photoreactive tRNA molecules as probes for cross-linking studies

Biochimie
J WowerR A Zimmermann

Abstract

Photoreactive tRNA derivatives have been used extensively for investigating the interaction of tRNA molecules with their ligands and substrates. Recombinant RNA technology facilitates the construction of such tRNA probes through site-specific incorporation of photoreactive nucleosides. The general strategy involves preparation of suitable tRNA fragments and their ligation either to a photoreactive nucleotide or to each other. tRNA fragments can be prepared by site-specific cleavage of native tRNAs, or synthesized by enzymatic and chemical means. A number of photoreactive nucleosides suitable for incorporation into tRNA are presently available. Joining of tRNA fragments is accomplished either by RNA ligase or by DNA ligase in the presence of a DNA splint. The application of this methodology to the study of tRNA binding sites on the ribosome is discussed, and a model of the tRNA-ribosome complex is presented.

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Citations

Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J WowerR A Zimmermann
Aug 7, 2012·Angewandte Chemie·Jan L VinkenborgMichael Famulok
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·K M Meisenheimer, T H Koch
Apr 20, 1999·Methods : a Companion to Methods in Enzymology·M M Konarska
May 23, 1998·Critical Reviews in Biochemistry and Molecular Biology·N BurkhardtK H Nierhaus

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