Recombinant protein detection in crude extracts by flow-injection immunoassay

Journal of Immunological Methods
Maria Paola Bartolomeo, Federico Maisano

Abstract

An immunofluorescence method that allows detection and quantification of recombinant proteins in complex mixtures (e.g. crude cell extracts and fermentation media) is described. The method is based on a non-denaturing chromatographic analysis which is performed after the addition of a fluorescently labelled antibody specific for the target protein. HPLC analysis through a size-exclusion column allows to separate and quantify the immunocomplex and the excess antibody, which are the only fluorescent species in the mixture. The method was applied to the analysis of recombinant human Fabs expressed in bacteria. Preliminary validation data are reported.

References

Jan 1, 1991·Journal of Pharmaceutical and Biomedical Analysis·P Fernandez-Hernando, J N Miller
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·D S Hage
Apr 26, 2001·Analytical Chemistry·D S Hage, M A Nelson

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Citations

Sep 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Emmanuel MatabaroByong H Lee

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