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Recombinant protein purification by self-cleaving elastin-like polypeptide fusion tag

Current Protocols in Protein Science

Nov 26, 2009

Wan-Yi WuDavid W Wood

Abstract

This unit presents a rapid and simple method for the nonchromatographic purification of recombinant proteins expressed in E. coli. This method relies on a thermally responsive elastin-like polypeptide (ELP) tag, where the tagged protein is precipitated using a mild temperature shift. Th...read more

Mentioned in this Paper

Shuttle Vectors
ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
expression vector
Endopeptidases
Proteolytic Enzyme
Protein Purification
Purification Aspects
Recombinants
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Recombinant protein purification by self-cleaving elastin-like polypeptide fusion tag

Current Protocols in Protein Science

Nov 26, 2009

Wan-Yi WuDavid W Wood

PMID: 19937722

DOI: 10.1002/0471140864.ps2604s58

Abstract

This unit presents a rapid and simple method for the nonchromatographic purification of recombinant proteins expressed in E. coli. This method relies on a thermally responsive elastin-like polypeptide (ELP) tag, where the tagged protein is precipitated using a mild temperature shift. Th...read more

Mentioned in this Paper

Shuttle Vectors
ELN gene
Alkalescens-Dispar Group
Chimeric Proteins, Recombinant
expression vector
Endopeptidases
Proteolytic Enzyme
Protein Purification
Purification Aspects
Recombinants

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