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Recombinant protein purification by self-cleaving elastin-like polypeptide fusion tag

Current Protocols in Protein Science

Nov 1, 2009

Wan-Yi WuDavid W Wood

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Abstract

This unit presents a rapid and simple method for the nonchromatographic purification of recombinant proteins expressed in E. coli. This method relies on a thermally responsive elastin-like polypeptide (ELP) tag, where the tagged protein is precipitated using a mild temperature shift. Th...read more

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  • Recombinant protein purification by self-cleaving elastin-like polypeptide fusion tag

    Current Protocols in Protein Science

    Nov 1, 2009

    Wan-Yi WuDavid W Wood

    PMID: 19937722

    DOI: 10.1002/0471140864.ps2604s58

    Abstract

    This unit presents a rapid and simple method for the nonchromatographic purification of recombinant proteins expressed in E. coli. This method relies on a thermally responsive elastin-like polypeptide (ELP) tag, where the tagged protein is precipitated using a mild temperature shift. Th...read more

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