Recombinant Pvs48/45 antigen expressed in E. coli generates antibodies that block malaria transmission in Anopheles albimanus mosquitoes

PloS One
Myriam Arévalo-HerreraSócrates Herrera

Abstract

Transmission of malaria parasites from humans to Anopheles mosquitoes can be inhibited by specific antibodies elicited during malaria infection, which target surface Plasmodium gametocyte/gamete proteins. Some of these proteins may have potential for vaccine development. Pvs48/45 is a P. vivax gametocyte surface antigen orthologous to Pfs48/45, which may play a role during parasite fertilization and thus has potential for transmission blocking (TB) activity. Here we describe the expression of a recombinant Pvs48/45 protein expressed in Escherichia coli as a ∼60kDa construct which we tested for antigenicity using human sera and for its immunogenicity and transmission blocking activity of specific anti-mouse and anti-monkey Pvs48/45 antibodies. The protein reacted with sera of individuals from malaria-endemic areas and in addition induced specific IgG antibody responses in BALB/c mice and Aotus l. griseimembra monkeys. Sera from both immunized animal species recognized native P. vivax protein in Western blot (WB) and immunofluorescence assays. Moreover, sera from immunized mice and monkeys produced significant inhibition of parasite transmission to An. Albimanus mosquitoes as shown by membrane feeding assays. Results indicate the...Continue Reading

References

Jan 1, 1992·Memórias do Instituto Oswaldo Cruz·K N Mendis, R Carter
Jul 31, 1998·Annals of Tropical Medicine and Parasitology·J K Baird
Aug 11, 1998·The Journal of Infectious Diseases·J K BairdS L Hoffman
Nov 6, 2004·Nature Biotechnology·François Baneyx, Mirna Mujacic
Nov 18, 2005·The American Journal of Tropical Medicine and Hygiene·Myriam Arévalo-HerreraSócrates Herrera
Nov 18, 2005·The American Journal of Tropical Medicine and Hygiene·Myriam Arévalo-HerreraSócrates Herrera
Nov 18, 2005·The American Journal of Tropical Medicine and Hygiene·Sócrates HerreraMyriam Arévalo-Herrera
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Joanna Kubler-KielbRachel Schneerson
Mar 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nikolay S OutchkourovHendrik G Stunnenberg
Oct 10, 2008·Nature·Jane M CarltonClaire M Fraser-Liggett
Jan 13, 2009·Clinical Microbiology Reviews·Denise L DoolanJ Kevin Baird
Feb 17, 2009·Nature Reviews. Microbiology·Arnab Pain, Christiane Hertz-Fowler
Aug 12, 2009·Molecular and Biochemical Parasitology·Duk-Won Doug ChungKarine G Le Roch
Aug 22, 2009·The Lancet Infectious Diseases·Ivo MuellerHernando A del Portillo
Feb 15, 2011·The American Journal of Tropical Medicine and Hygiene·Sócrates HerreraMyriam Arévalo-Herrera
Feb 15, 2011·The American Journal of Tropical Medicine and Hygiene·Myriam Arévalo-HerreraSócrates Herrera
Aug 1, 2012·Bioinformatics·Paulo GasparGabriela Moura

❮ Previous
Next ❯

Citations

Sep 13, 2016·Biotechnology and Bioengineering·Gina R WebsterJulian K-C Ma
Dec 17, 2019·Immunological Reviews·Roos M de JongTeun Bousema
Jun 30, 2016·PLoS Neglected Tropical Diseases·Andrés F VallejoMyriam Arévalo-Herrera
May 8, 2018·Parasites & Vectors·Keillen M Martins-CamposMaria das Graças V Barbosa Guerra
Jan 10, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Soheila AsaliShahram Solaymani-Mohammadi
Dec 3, 2016·International Journal for Parasitology·Silvia A Arredondo, Stefan H I Kappe
Nov 9, 2021·Parasitology International·Sirasate BantuchaiWang Nguitragool

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
electrophoresis
PCR
reverse-phase chromatography
enzyme-linked immunosorbent assay
ELISA
sedation
Assay

Software Mentioned

GPI
Bioedit sequence alignment editor
SMART ( SimpleModular Architecture Research Tools )
Scaffold
Geneious
EuGene
MASCOT
Pfam
SOM
Signal P

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Anorexia Nervosa

Anorexia nervosa (AN) is a psychiatric condition characterized by severe weight loss and secondary problems associated with malnutrition. Here is the latest research on AN.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.