Recombinant Sendai virus vectors deleted in both the matrix and the fusion genes: efficient gene transfer with preferable properties

The Journal of Gene Medicine
Makoto InoueMamoru Hasegawa

Abstract

Sendai virus (SeV) is a new type of cytoplasmic RNA vector, which infects and replicates in most mammalian cells, directs high-level expression of the genes on its genome and is free from genotoxicity. In order to improve this vector, both the matrix (M) and fusion (F) genes were deleted from its genome. For the recovery of the M and F genes-deleted SeV (SeV/DeltaMDeltaF), the packaging cell line was established by using a Cre/loxP induction system. SeV/DeltaMDeltaF was characterized and compared with wild-type and F or M gene-deleted SeV vectors in terms of transduction ability, particle formation, transmissible property and cytotoxicity. SeV/DeltaMDeltaF was propagated in high titers from the packaging cell line. When this vector was administered into the lateral ventricle and the respiratory tissue, many of the ependymal and epithelial cells were transduced, respectively, as in the case of wild-type SeV. F gene-deletion made the SeV vector non-transmissible, and M gene-deletion worked well to inhibit formation of the particles from infected cells. Simultaneous deletions of these two genes in the same genome resulted in combining both advantages. That is, both virus maturation into particles and transmissible property were al...Continue Reading

References

Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T R FuerstB Moss
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M A MarkwellJ C Paulson
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M H HeggenessP W Choppin
Jun 1, 1996·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·A KatoY Nagai
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·H GaroffD J Opstelten
Sep 6, 2000·Nature Biotechnology·Y YonemitsuE W Alton
May 10, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I MasakiK Sueishi
Jun 22, 2002·Virus Research·Tsuyoshi TokusumiMamoru Hasegawa
Jun 29, 2002·Journal of Virological Methods·Takahiro HirataMamoru Hasegawa
Jan 17, 2003·The New England Journal of Medicine·Salima Hacein-Bey-AbinaAlain Fischer

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Citations

Dec 22, 2009·Acta Neuropathologica·Marc R Del Bigio
Aug 2, 2006·Biological & Pharmaceutical Bulletin·Shigeo FujitaMahito Nakanishi
Apr 26, 2014·World Journal of Gastroenterology : WJG·Kazuyuki MatsushitaFumio Nomura
Jul 6, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Juliane W SchottAxel Schambach
Jun 29, 2007·Gene Therapy·S FerrariE W F W Alton
Feb 1, 2007·Audiology & Neuro-otology·Sho KanzakiKaoru Ogawa
Jul 12, 2007·The Journal of Biological Chemistry·Ken NishimuraMahito Nakanishi
May 18, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yasuo IwadateHideaki Shimada

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