PMID: 8613634Mar 1, 1996Paper

Recombinant soluble form of the human high-affinity receptor for IgE prevents anaphylactic shock in mice

The Journal of Allergy and Clinical Immunology
K NaitoC Ra

Abstract

The ectodomain of the alpha subunit of the human high-affinity receptor for IgE (Fc epsilon RI) has been engineered as a recombinant soluble protein (sFc epsilon RI alpha) and purified in large quantity from the supernatant of transfected mammalian cells. The sFc epsilon RI alpha was apparently heterogeneous in terms of molecular weight (40 to 60 kd), and this heterogeneity was largely due to N-linked carbohydrates on the molecule. The amino terminal sequence was homogeneous and identical to the sequence predicted from the complementary DNA sequence. The amino acid composition of the sFc epsilon RI alpha was in agreement with the values expected from the cDNA sequence. The sFc epsilon RI alpha formed a complex with IgE, and gel filtration analyses of the complex of the sFc epsilon RI alpha and human IgE supported the idea that the stoichiometry of the IgE binding was 1:1. Passive anaphylactic shock in mice was suppressed when the sFc epsilon RI alpha was intravenously injected after sensitization with anti-trinitrophenyl IgE and challenged with the appropriate antigen. In this model the sFc epsilon RI alpha was effective when administered 48 hours before the antigen challenge, but not 24 hours before the challenge. These result...Continue Reading

Citations

Jun 8, 2002·Advanced Drug Delivery Reviews·Kenneth D Hinds, Sung Wan Kim
Feb 13, 2008·International Archives of Allergy and Immunology·Barbara ProustGisèle Kanny
May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B M JensenL K Poulsen
May 9, 2014·Allergology International : Official Journal of the Japanese Society of Allergology·Reiko ItoShu Hashimoto
Jun 24, 2000·Clinical Immunology : the Official Journal of the Clinical Immunology Society·M YanagidaC Ra
May 16, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kun LinLi Li

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