PMID: 2492108Jan 1, 1989Paper

Recombinant system for overexpression of cholera toxin B subunit in Vibrio cholerae as a basis for vaccine development

Proceedings of the National Academy of Sciences of the United States of America
J Sanchez, J Holmgren

Abstract

We have constructed an overexpression system in which the gene encoding the B subunit of cholera toxin (CTB) was placed under the control of the strong tacP promoter in a wide host range plasmid. Recombinant nontoxigenic classical and E1 Tor Vibrio cholerae strains of different serotypes harboring this plasmid excreted 10- to 100-fold higher amounts of CTB than any other wild-type or recombinant strain tested and may therefore be useful killed oral vaccine strains. The manipulations to place the CTB gene under tacP also included, by design, the introduction of single enzyme restriction sites for gene fusions to the CTB amino terminus. Cloning into these sites allows construction of CTB-derived hybrid proteins carrying various putative vaccine peptide antigens.

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Citations

Aug 27, 1991·Biochimica Et Biophysica Acta·E DamsW Dierick
Jan 1, 1992·Vaccine·J HolmgrenH Wigzell
Aug 30, 1991·Gene·D K PandaJ Das
Sep 1, 1990·Research in Microbiology·J SanchezJ Holmgren
Mar 31, 1998·Journal of Immunological Methods·S LiljeqvistM Murby
Jan 27, 1999·Toxicon : Official Journal of the International Society on Toxinology·B L van BaarE R Wils
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T D BartleyW N Burnette
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·J B SunC Czerkinsky
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·J B SunC Czerkinsky
Oct 25, 2000·Journal of Pediatric Gastroenterology and Nutrition·M M Levine
Nov 24, 1999·Immunological Reviews·C CzerkinskyJ B Sun
Jan 26, 2013·PloS One·John BenktanderSusann Teneberg
Jul 9, 2009·Bioscience, Biotechnology, and Biochemistry·Takeshi MatsuiKo Kato
Apr 1, 1989·Vaccine·J HolmgrenA M Svennerholm
Dec 10, 2008·Journal of Molecular Biology·Ulrich SchroederCora-Ann Schoenenberger

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