PMID: 9557697Apr 29, 1998Paper

Recombinant vaccinia virus coexpressing the F protein of respiratory syncytial virus (RSV) and interleukin-4 (IL-4) does not inhibit the development of RSV-specific memory cytotoxic T lymphocytes, whereas priming is diminished in the presence of high levels of IL-2 or gamma interferon

Journal of Virology
G P BembridgeG Taylor

Abstract

In order to investigate if immune responses to the fusion (F) protein of respiratory syncytial virus (RSV) could be influenced by cytokines, recombinant vaccinia viruses (rVV) carrying both the F gene of RSV and the gene for murine interleukin-2 (IL-2), IL-4, or gamma interferon (IFN-gamma) were constructed. In vitro characterization of rVV revealed that insertion of the cytokine gene into the VP37 locus of the vaccinia virus genome resulted in 100- to 1,000-fold higher expression than insertion of the same gene into the thymidine kinase (TK) locus. In comparison, only a two- to fivefold difference in the level of expression of the F protein was observed when the gene was inserted into either of these two loci. Mice vaccinated with rVV expressing the F protein and high levels of IL-2 or IFN-gamma cleared rVV more rapidly than mice inoculated with a control rVV and developed only low levels of RSV-specific serum antibody. In addition, these recombinants were much less effective at priming RSV-specific memory cytotoxic T lymphocytes (CTL) and IFN-gamma production by spleen cells than rVV expressing the F protein alone. In contrast, mice vaccinated with rVV expressing high levels of IL-4 showed signs of delayed rVV clearance. RSV-...Continue Reading

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Citations

Oct 20, 2000·The Journal of General Virology·C RaggoP Griebel
Jul 16, 2005·Expert Opinion on Biological Therapy·Martin Cranage, Geraldine Taylor
Feb 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Norbert KienzleAnne Kelso
Sep 23, 2006·Expert Review of Vaccines·Emma L BeukemaJohn D Hayball
Aug 30, 2012·Immunology and Cell Biology·Carolina JohnstoneMargarita Del Val
Jun 20, 2020·Journal of Animal Physiology and Animal Nutrition·Ligang XueWeimin Luan
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Norbert KienzleAnne Kelso
May 29, 2004·The Journal of General Virology·Nathan W BartlettGeoffrey L Smith
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·A BukreyevP L Collins
Aug 3, 2007·Expert Review of Vaccines·Cara K FraserJohn D Hayball
Dec 3, 2013·Archives of Virology·Wendy FonsecaYoshihiro Kawaoka
Mar 11, 2005·Expert Review of Vaccines·Michael A SkinnerMatthew G Cottingham
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J ChangT J Braciale
Feb 5, 2005·Veterinary Immunology and Immunopathology·Susanne Nedergaard GrellPeter Mikael Helweg Heegaard

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