Recombination and selectional forces in cyanopeptolin NRPS operons from highly similar, but geographically remote Planktothrix strains.

BMC Microbiology
Trine B RoungeKjetill S Jakobsen

Abstract

Cyanopeptolins are nonribosomally produced heptapetides showing a highly variable composition. The cyanopeptolin synthetase operon has previously been investigated in three strains from the genera Microcystis, Planktothrix and Anabaena. Cyanopeptolins are displaying protease inhibitor activity, but the biological function(s) is (are) unknown. Cyanopeptolin gene cluster variability and biological functions of the peptide variants are likely to be interconnected. We have investigated two cyanopeptolin gene clusters from highly similar, but geographically remote strains of the same genus. Sequencing of a nonribosomal peptide synthetase (NRPS) cyanopeptolin gene cluster from the Japanese strain Planktothrix NIES 205 (205-oci), showed the 30 kb gene cluster to be highly similar to the oci gene cluster previously described in Planktothrix NIVA CYA 116, isolated in Norway. Both operons contained seven NRPS modules, a sulfotransferase (S) and a glyceric acid loading (GA)-domain. Sequence analyses showed a high degree of conservation, except for the presence of an epimerase domain in NIES 205 and the regions around the epimerase, showing high substitution rates and Ka/Ks values above 1. The two strains produce almost identical cyanopept...Continue Reading

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Citations

Sep 21, 2012·ACS Chemical Biology·Jennifer Gehret McCarthyJanet L Smith
Dec 21, 2010·Applied and Environmental Microbiology·Jørn Henrik Sønstebø, Thomas Rohrlack
Apr 23, 2013·BMC Evolutionary Biology·Tânia Keiko ShishidoKaarina Sivonen
Oct 1, 2018·Journal of Industrial Microbiology & Biotechnology·Marc G Chevrette, Cameron R Currie
Jan 23, 2021·Molecular Biology and Evolution·Martin BaunachElke Dittmann

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Datasets Mentioned

BETA
EU109504
DQ837301
DQ075244
AJ269505

Methods Mentioned

BETA
sulfotransferase
gene knock-out
PCR

Software Mentioned

HighChemMassFrontier
MEGA
MrBayes
RAxML
DnaSP

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