Recombination between DNA repeats in yeast hpr1delta cells is linked to transcription elongation

The EMBO Journal
Félix PradoAndrés Aguilera

Abstract

The induction of recombination by transcription activation has been documented in prokaryotes and eukaryotes. Unwinding of the DNA duplex, disruption of chromatin structure or changes in local supercoiling associated with transcription can be indirectly responsible for the stimulation of recombination. Here we provide genetic and molecular evidence for a specific mechanism of stimulation of recombination by transcription. We show that the induction of deletions between repeats in hpr1delta cells of Saccharomyces cerevisiae is linked to transcription elongation. Molecular analysis of different direct repeat constructs reveals that deletions induced by hpr1delta are specific for repeat constructs in which transcription initiating at an external promoter traverses particular regions of the DNA flanked by the repeats. Transcription becomes HPR1 dependent when elongating through such regions. Both the induction of deletions and the HPR1 dependence of transcription were abolished when a strong terminator was used to prevent transcription from proceeding through the DNA region flanked by the repeats. In contrast to previously reported cases of transcription-induced recombination, there was no correlation between high levels of transcr...Continue Reading

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Citations

Oct 26, 2007·Molecular Genetics and Genomics : MGG·María García-RubioAndrés Aguilera
Sep 28, 1998·Biochimica Et Biophysica Acta·J L Nitiss
Jul 8, 1999·Current Opinion in Cell Biology·D ReinesJ W Conaway
Mar 6, 1999·Current Opinion in Microbiology·A IshihamaH Mitsuzawa
Apr 20, 2013·Chemical Reviews·Hélène GaillardAndrés Aguilera
Aug 1, 2008·Molecular Biology of the Cell·Cristina González-AguileraAndrés Aguilera
Feb 2, 2002·The EMBO Journal·Andrés Aguilera
May 10, 2002·Nucleic Acids Research·Sergio González-BarreraAndrés Aguilera
Jan 27, 2012·Genes & Development·Peter C StirlingPhilip Hieter
Aug 5, 2009·Molecular and Cellular Biology·Belén Gómez-GonzálezAndrés Aguilera
Oct 31, 2007·Molecular and Cellular Biology·Ponnari GottipatiThomas Helleday
Jun 8, 2001·Molecular and Cellular Biology·D Kulish, K Struhl
Jan 5, 2002·Molecular and Cellular Biology·Robert J Merker, Hannah L Klein
Nov 26, 2002·Molecular and Cellular Biology·Robert J Merker, Hannah L Klein
Feb 3, 2005·Molecular and Cellular Biology·Félix Prado, Andrés Aguilera
Sep 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Vladimir VoynovGerald R Fink
May 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·Belén Gómez-González, Andrés Aguilera
Jan 30, 2008·Nature Reviews. Genetics·Andrés Aguilera, Belén Gómez-González
Jan 8, 2011·Molecular Cell·Hannah E MischoNick J Proudfoot
Mar 30, 2016·Annual Review of Biochemistry·Hélène Gaillard, Andrés Aguilera
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Francisco García-BenítezAndrés Aguilera
Oct 19, 2017·The Journal of Cell Biology·Emily Yun-Chia ChangPeter C Stirling
Jul 23, 2003·The Journal of Biological Chemistry·Ana G RondónAndrés Aguilera
Jan 15, 2004·European Journal of Human Genetics : EJHG·Markus Hildinger, Alberto Auricchio

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