Recombination creates novel L1 (LINE-1) elements in Rattus norvegicus.

Genetics
B E HaywardA V Furano

Abstract

Mammalian L1 (long interspersed repeated DNA. LINE-1) retrotransposons consist of a 5' untranslated region (UTR) with regulatory properties, two protein encoding regions (ORF I, ORF II, which encodes a reverse transcriptase) and a 3' UTR. L1 elements have been evolving in mammals for > 100 million years and this process continues to generate novel L1 subfamilies in modern species. Here we characterized the youngest known subfamily in Rattus norvegicus, L1mlvi2, and unexpectedly found that this element has a dual ancestry. While its 3' UTR shares the same lineage as its nearest chronologically antecedent subfamilies, L13 and L14, its ORF I sequence does not. The L1mlvi2 ORF I was derived from an ancestral ORF I sequence that was the evolutionary precursor of the L13 and L14 ORF I. We suggest that an ancestral ORF I sequence was recruited into the modern L1mlvi2 subfamily by recombination that possibly could have resulted from template strand switching by the reverse transcriptase during L1 replication. This mechanism could also account for some of the structural features of rodent L1 5' UTR and ORF I sequences including one of the more dramatic features of L1 evolution in mammals, namely the repeated acquisition of novel 5' UTRs.

Citations

Dec 28, 2005·Biochemical and Biophysical Research Communications·Anton V FedorovOlga I Podgornaya
May 24, 2021·Nucleic Acids Research·Emily C StowVictoria P Belancio
Jan 5, 2013·Mobile DNA·Akash SookdeoStéphane Boissinot
Dec 13, 2007·Nucleic Acids Research·Alexander KirilyukGerald G Schumann
Feb 20, 2007·Human Mutation·Daria V Babushok, Haig H Kazazian
Aug 12, 2005·Cytogenetic and Genome Research·S L MartinS Boissinot
Aug 12, 2005·Cytogenetic and Genome Research·N ZinglerG G Schumann
Aug 12, 2005·Cytogenetic and Genome Research·A BuzdinE Sverdlov
Sep 16, 2020·Brain Research·Amelia CuarentaAnthony P Auger
Aug 19, 2005·Molecular and Cellular Biology·Nicolas GilbertJohn V Moran
Aug 1, 2006·Journal of Biomedicine & Biotechnology·Sandra L Martin
Jun 15, 2007·FEBS Letters·Anton BuzdinMarc-Henri Lebrun
Oct 8, 1997·Journal of Molecular Evolution·E L CabotA V Furano
Apr 2, 2004·Nature·Richard A GibbsUNKNOWN Rat Genome Sequencing Project Consortium
Nov 23, 2019·Genome Biology and Evolution·Hajk-Georg Drost, Diego H Sanchez
Feb 7, 2001·Molecular and Cellular Biology·W WeiJ V Moran
Aug 15, 2002·Cell·Nicolas GilbertJohn V Moran
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·O VerneauA V Furano
Aug 4, 2009·Cellular and Molecular Life Sciences : CMLS·Elena Gogvadze, Anton Buzdin
Oct 10, 2001·Genome Research·J L GoodierH H Kazazian
Dec 21, 2005·Genome Research·Daria V BabushokHaig H Kazazian

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