Recombination of mitochondrial DNAs following transmission of mitochondria among incompatible strains of black Aspergilli

Molecular & General Genetics : MGG
F KeveiJ H Croft

Abstract

Successful intra- and interspecific mitochondrial transfers were performed by polyethylene glycol (PEG)-induced protoplast fusion among incompatible strains belonging to the Aspergillus niger species aggregate. The mitochondrial DNAs (mtDNAs) of the strains examined were of three main types based on their restriction fragment length polymorphism (RFLP) profiles. mtDNA types 1 and 2 correspond to A. niger and A. tubingensis species, respectively, while type 3 is represented by some Brazilian wild-type isolates (possibly a distinct species or subspecies). mtDNA types 1 and 2 could be further divided into several subgroups (1a-1e and 2a-2f). All these strains, representing different RFLP groups or subgroups, were fully incompatible with respect to nuclear complementation. The transfer experiments were carried out under selection pressure, using a mitochondrial oligomycin-resistant mutant of mtDNA type 1a as donor. Following fusion mitochondrial oligomycin-resistant progenies were recovered in the presence of oligomycin by selecting for the nuclear phenotypes of the oligomycin-sensitive recipient strains. All attempted transfers were successful, and resulted in different varieties of resistant recombinant mitochondrial progenies at...Continue Reading

Citations

Aug 15, 2014·Mycopathologia·János VargaPaul S Dyer
Jun 28, 2001·Acta Biologica Hungarica·K SwartR F Hoekstra
May 21, 2008·Studies in Mycology·D M GeiserR A Samson
Jul 12, 2002·Acta Microbiologica Et Immunologica Hungarica·Zsuzsanna HamariF Kevei

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