Recommendations for appropriate activated partial thromboplastin time reagent selection and utilization

American Journal of Clinical Pathology
G FritsmaEllinor I Peerschke

Abstract

The activated partial thromboplastin time (aPTT) is widely used as a screening coagulation test and for monitoring unfractionated heparin therapy. Various commercial reagents are available, with different performance characteristics, particularly responsiveness to the lupus anticoagulant (LA). Because aPTT reagent selection significantly affects the interpretation of results, we reviewed College of American Pathologists proficiency testing data involving approximately 4,000 coagulation laboratories, and conducted a survey of coagulation laboratories (n = 93) using The Fritsma Factor hemostasis Web site to determine the basis for aPTT reagent selection. The data demonstrate that for routine aPTT testing, most laboratories use reagents with high/moderate responsiveness to LA. Significant misunderstanding was apparent regarding the use of appropriate aPTT reagent for routine testing and LA identification. We recommend aPTT reagents with low LA responsiveness to screen for coagulation factor deficiencies and heparin monitoring, and suggest continued education of laboratory professionals and reagent manufacturers about appropriate aPTT reagent use.

References

Dec 1, 1993·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·A M van den BesselaarR M Bertina
Jul 15, 1993·Annals of Internal Medicine·P Brill-EdwardsJ Hirsh
Feb 6, 1954·Journal of the American Medical Association·K M BRINKHOUSR H WAGNER
Feb 12, 2005·Seminars in Thrombosis and Hemostasis·Richard C W WongEmmanuel J Favaloro
Jul 25, 2009·Journal of Thrombosis and Haemostasis : JTH·V PengoUNKNOWN Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the Interna
Oct 21, 2010·American Journal of Clinical Pathology·Francine R DembitzerEllinor I B Peerschke
Jun 29, 2011·Journal of Thrombosis and Haemostasis : JTH·J SwadzbaJ Musial

❮ Previous
Next ❯

Citations

Aug 26, 2015·International Journal of Laboratory Hematology·G W MooreB J Hunt
May 15, 2013·American Journal of Clinical Pathology·Shirimati Shetty, Kanjaksha Ghosh
Sep 19, 2014·Thrombosis and Haemostasis·M Van BlerkP Van de Walle
Feb 18, 2015·Haemophilia : the Official Journal of the World Federation of Hemophilia·S KitchenN S Key
May 30, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Éva AjznerUNKNOWN joint Working Group on Postanalytical Phase (WG-POST) of the European Federation of Clinical Chemistry and Laboratory Medici
Mar 13, 2015·Thrombosis Journal·Herm Jan M Brinkman
May 18, 2016·International Journal of Laboratory Hematology·P ToulonA Appert-Flory
Jul 28, 2016·Journal of Cardiothoracic and Vascular Anesthesia·Ariane WillemsPhilippe Van der Linden
Dec 15, 2016·Analytical Chemistry·Francesco PadovaniMartin Hegner
Feb 28, 2018·Journal of Clinical Laboratory Analysis·Mesude FalayGülsüm Özet
Mar 8, 2016·Anesthesiology·Daniel DirkmannJürgen Peters
Apr 21, 2020·American Journal of Hematology·Gary W Moore
Mar 5, 2019·Diagnosis·Giuseppe LippiEmmanuel J Favaloro
Dec 2, 2016·Antibodies·Gary W Moore
Jul 27, 2018·Research and Practice in Thrombosis and Haemostasis·Gary W MooreMaria L Bertolaccini
Dec 4, 2013·ACS Applied Materials & Interfaces·Liang-Cheng SuMei-Chin Chen
Sep 24, 2021·Nature Communications·Marco HeestermansThomas Renné

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Related Papers

American Journal of Clinical Pathology
Liron PantanowitzLynne Uhl
Joint Commission Journal on Quality and Patient Safety
Edward L MachtingerDean Schillinger
Clinical Laboratory Science : Journal of the American Society for Medical Technology
G Fritsma
The American Journal of Psychiatry
A ShanerS C Hsieh
© 2021 Meta ULC. All rights reserved