PMID: 7514739Jun 1, 1994Paper

Recommendations for the performance of UDS tests in vitro and in vivo

Mutation Research
S MadleH Mori

Abstract

The Working Group (WG) dealt with the harmonization of routine methodologies of tests for unscheduled DNA synthesis (UDS) both in vitro and in vivo. In contrast to the existing guidelines from OECD, EPA and EC on in vitro UDS tests (there is no Japanese UDS guideline), the Working Group recommends that in general in vitro UDS tests should be performed with primary hepatocytes. For routine applications any other cell types would need special justification. Hepatocytes from male rats are preferable, unless there are contra-indications on the basis of e.g. toxicokinetic data. According to the OECD, EPA and EC guidelines, UDS may be analysed by means of autoradiography (AR) or liquid scintillation counting (LSC). The WG recommends use of AR. LSC is less suitable due to the problem of differentiation between UDS activity and replicative DNA synthesis, and the disadvantage that cells cannot be analysed individually. Since a specific cell type was recommended by the WG, methodological aspects could be described in more detail than in the present guidelines. For in vitro tests, it was agreed that the initial viability of freshly isolated hepatocytes should be at least 70%. With regard to the need for confirmatory experiments in the eve...Continue Reading

References

Jan 1, 1992·Teratogenesis, Carcinogenesis, and Mutagenesis·A AllavenaG Brambilla
Aug 1, 1992·Mutation Research·G Brambilla, A Martelli
Jan 1, 1992·Environmental and Molecular Mutagenesis·B E ButterworthM E Hurtt
Mar 1, 1991·Chemical Research in Toxicology·F P GuengerichM Iwasaki
Jan 1, 1991·Environmental and Molecular Mutagenesis·C A McQueen, B M Way
Jan 1, 1990·Environmental and Molecular Mutagenesis·P SchmezerJ Ashby
Jan 1, 1989·Environmental and Molecular Mutagenesis·J AshbyL Bandara
Dec 1, 1989·Carcinogenesis·E GeorgeD Gatehouse
Jun 1, 1987·Cell Biology and Toxicology·T R BarfknechtD J Kornbrust
Jun 1, 1987·Cell Biology and Toxicology·C A McQueen, G M Williams
May 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D J DoolittleH E Scribner
Jan 1, 1987·Environmental and Molecular Mutagenesis·R W Trueman, J Ashby
Dec 1, 1986·Mutation Research·J AshbyB Beije
Apr 1, 1985·Mutation Research·J AshbyM G Penman
Oct 1, 1972·Chemico-biological Interactions·W N BrandtN J Bernheim
May 1, 1982·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·C A McQueen, G M Williams
Oct 1, 1983·Mutation Research·B E ButterworthG Michalopoulos

❮ Previous
Next ❯

Citations

May 3, 1996·International Journal of Cancer. Journal International Du Cancer·H J CrossA Gescher
Feb 18, 2011·Archives of Toxicology·Giovanni BrambillaAntonietta Martelli
Dec 1, 1995·Mutation Research·K Müller-TegethoffL Müller
Nov 25, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gerhard J NohynekHiroyuki Jinnai
Aug 1, 1997·Mutation Research·P M Eckl, I Raffelsberger
Jan 16, 2003·Virus Research·Esteban DomingoFrancisco Sobrino
Apr 14, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A J EdwardsB G Lake
Aug 29, 2001·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·B G LakeT B Adams
May 7, 2010·Analytical and Bioanalytical Chemistry·Valérie Fessard, Ludovic Le Hégarat
May 29, 2004·Mutation Research·John WhysnerElizabeth A Lax
Oct 22, 2010·Environmental and Molecular Mutagenesis·Kerry L DearfieldKristine L Witt
Feb 16, 2005·Toxicology and Applied Pharmacology·Francesca MattioliGiovanni Brambilla
Aug 6, 2005·Mutation Research·Iain B LambertGeorge R Douglas
Jul 28, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Cheryl A HobbsKristine L Witt
Jul 28, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Takeshi MoritaLeonard Schechtman
Jun 19, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Awais IhsanZonghui Yuan
Oct 16, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Awais IhsanZong-Hui Yuan
Sep 14, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Alexander Cartus, Dieter Schrenk
Sep 1, 2007·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Mary K ManibusanDavid A Eastmond
Sep 28, 2010·Critical Reviews in Toxicology·Richard J AlbertiniMichael L Gargas
May 1, 2001·Current Protocols in Toxicology·J E Sutherland, M Costa
Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Vanessa ValdiglesiasBlanca Laffon
Mar 11, 2020·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Martyn T SmithMark Fielden

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.