Reconciling the controversy regarding the functional importance of bullet- and football-shaped GroE complexes

The Journal of Biological Chemistry
Lavi S Bigman, Amnon Horovitz

Abstract

The chaperonin GroEL and its co-chaperonin GroES form both GroEL-GroES bullet-shaped and GroEL-GroES2 football-shaped complexes. The residence time of protein substrates in the cavities of these complexes is about 10 and 1 s, respectively. There has been much controversy regarding which of these complexes is the main functional form. Here, we show using computational analysis that GroEL protein substrates have a bimodal distribution of folding times, which matches these residence times, thereby suggesting that both bullet-shaped and football-shaped complexes are functional. More generally, co-existing complexes with different stoichiometries are not mutually exclusive with respect to having a functional role and can complement each other.

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Citations

Jun 26, 2020·Frontiers in Molecular Biosciences·Celeste Caruso BavisottoAntonella Marino Gammazza
Apr 23, 2020·Nature Communications·Yacob Gomez-LlorenteIban Ubarretxena-Belandia
Jun 16, 2021·The Journal of Physical Chemistry Letters·Nicolas MacroDongping Zhong

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