Reconfigurable microfluidic device with integrated antibody arrays for capture, multiplexed stimulation, and cytokine profiling of human monocytes

Biomicrofluidics
Tam VuAlexander Revzin

Abstract

Monocytes represent a class of immune cells that play a key role in the innate and adaptive immune response against infections. One mechanism employed by monocytes for sensing foreign antigens is via toll-like receptors (TLRs)-transmembrane proteins that distinguish classes of foreign pathogens, for example, bacteria (TLR4, 5, and 9) vs. fungi (TLR2) vs. viruses (TLR3, 7, and 8). Binding of antigens activates a signaling cascade through TLR receptors that culminate in secretion of inflammatory cytokines. Detection of these cytokines can provide valuable clinical data for drug developers and disease investigations, but this usually requires a large sample volume and can be technically inefficient with traditional techniques such as flow cytometry, enzyme-linked immunosorbent assay, or luminex. This paper describes an approach whereby antibody arrays for capturing cells and secreted cytokines are encapsulated within a microfluidic device that can be reconfigured to operate in serial or parallel mode. In serial mode, the device represents one long channel that may be perfused with a small volume of minimally processed blood. Once monocytes are captured onto antibody spots imprinted into the floor of the device, the straight channe...Continue Reading

References

Aug 1, 1994·European Journal of Immunology·H W Ziegler-HeitbrockM Ströbel
Feb 1, 1997·European Journal of Immunology·F ChapuisJ C Gluckman
Jun 1, 1997·Journal of Leukocyte Biology·P Antal-SzalmasK P Van Kessel
Aug 5, 1998·Lancet·A Barnes
Dec 16, 1998·Research in Immunology·R van Furth
Nov 5, 1999·International Immunology·F D Finkelman, S C Morris
Jul 14, 2001·Trends in Biotechnology·J L Ding, B Ho
Nov 1, 2001·Clinical and Diagnostic Laboratory Immunology·M KouwenhovenH Link
Oct 25, 2002·Diabetes/metabolism Research and Reviews·Guido MeierhoffNanette C Schloot
Jan 31, 2004·Seminars in Immunology·Kiyoshi Takeda, Shizuo Akira
Mar 23, 2004·Journal of Dermatological Science·Kiyoshi Takeda, Shizuo Akira
Sep 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tin Ky MaoM Eric Gershwin
Jul 11, 2006·Journal of Immunological Methods·Kazuhiko SekineMehmet Toner
Jan 5, 2007·Lab on a Chip·Kathryn HaubertDavid Beebe
Apr 24, 2007·Cytokine·Gitika PanickerElizabeth R Unger
Nov 22, 2008·Lab on a Chip·He ZhuAlexander Revzin
Aug 12, 2009·Brain, Behavior, and Immunity·Amanda M EnstromPaul Ashwood
Sep 11, 2009·Analytical Chemistry·He ZhuAlexander Revzin
Apr 9, 2010·Journal of Innate Immunity·Arti PariharAndrea I Doseff
Dec 1, 1998·Analytical Chemistry·D C DuffyG M Whitesides
Jun 28, 2011·BMC Bioinformatics·Christopher R BolenSteven H Kleinstein
Oct 12, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Nicole BrooksDodie S Pouniotis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.