Reconsideration of the proposed luteotropic and luteoprotective actions of ovine placental lactogen in sheep: in vivo and in vitro studies

The Journal of Endocrinology
Kaïs H Al-GuboryIrène Ceballos-Picot

Abstract

Ovine placental lactogen (oPL) is produced by the conceptus trophectoderm and is secreted into both the maternal and fetal circulations. The present study was designed to examine in vivo the luteotropic effect of recombinant oPL (roPL), as determined by monitoring progesterone concentration and cycle length (experiment 1), and the antioxidative and antiapoptotic effects of roPL, as determined respectively by monitoring antioxidant enzymatic activity and apoptosis in the corpus luteum (CL) of cyclic ewes (experiment 2). We also studied whether roPL is capable of stimulating progesterone secretion in vitro by cultured luteal tissue of functionally active CL obtained from day-10 cyclic ewes (experiment 3) and day-60 pregnant ewes (experiment 4). Circulating concentrations of progesterone and cycle length were not affected by treatment of ewes with 80 microg/kg body weight per day of roPL (n = 4 ewes) for 10 days beginning on day 11 post-estrus, as compared with saline-treated ewes (n = 4 ewes). Luteolysis occurred between days 15 and 16 post-estrus in the four saline-treated ewes and in 3/4 roPL-treated ewes. The activities of the key antioxidant enzymes copper-zinc superoxide dismutase (Cu,Zn-SOD), manganese SOD (Mn-SOD), glutath...Continue Reading

Citations

Oct 27, 2007·BMC Molecular Biology·Koichi UshizawaKazuyoshi Hashizume
Oct 16, 2012·Reproductive Biomedicine Online·Kaïs H Al-GuboryNorihiro Sugino
Jul 6, 2010·The International Journal of Biochemistry & Cell Biology·Kaïs H Al-GuboryCatherine Garrel
May 31, 2008·Endocrinology·Kaïs H Al-GuboryCatherine Garrel
Feb 27, 2020·International Journal of Molecular Sciences·Caroline EozenouOlivier Sandra
Nov 25, 2020·Zygote : the Biology of Gametes and Early Embryos·Hussein M El-ZaherMostafa S A Khattab

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