PMID: 8953378Dec 1, 1996Paper

Reconstituted Cl- pump protein: a novel ion(Cl-)-motive ATPase

Journal of Bioenergetics and Biomembranes
George A Gerencser, K R Purushotham

Abstract

Cl- absorption by the Aplysia californica foregut is effected through an active Cl- transport mechanism located in the basolateral membrane of the epithelial absorptive cells. These basolateral membranes contain both Cl(-)-stimulated ATPase and ATP-dependent Cl- transport activities which can be incorporated into liposomes via reconstitution. Utilizing the proteoliposomal preparation, it was demonstrated that ATP, and its subsequent hydrolysis, Mg2+, Cl-, and a pH optimum of 7.8 were required to generate maximal intraliposomal Cl- accumulation, electrical negativity, and ATPase activity. Additionally, an inwardly-directed valinomycin-induced K+ diffusion potential, making the liposome interior electrically positive, enhanced both ATP-driven Cl- accumulation and electrical potential while an outwardly-directed valinomycin-induced K+ diffusion potential, making the liposome interior electrically negative, decreased both ATP-driven Cl- accumulation and electrical potential compared with proteoliposomes lacking the ionophore. Either orthovanadate or p-chloromercurobenzene sulfonate inhibited both the ATP-dependent intraliposomal Cl- accumulation, intraliposomal negative potential difference, and also Cl(-)-stimulated ATPase activit...Continue Reading

References

Dec 14, 1990·Biochimica Et Biophysica Acta·G A Gerencser
Jan 1, 1989·Annals of the New York Academy of Sciences·C L Slayman, G R Zuckier
Mar 1, 1985·The American Journal of Physiology·A PrigentM Paillard
Jul 15, 1988·Biochemical and Biophysical Research Communications·C Inagaki, T Shiroya
Jan 1, 1981·Physiological Reviews·F Schuurmans Stekhoven, S L Bonting
Sep 5, 1984·Biochimica Et Biophysica Acta·G A Gerencser
Oct 25, 1993·FEBS Letters·G A Gerencser
Nov 15, 1993·Biochemical and Biophysical Research Communications·G A Gerencser
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G A Gerencser, B Zelezna

❮ Previous
Next ❯

Citations

Jul 13, 2002·The Journal of Experimental Zoology·Georpe A Gerencser, Jianliang Zhang
May 15, 2001·The Journal of Experimental Zoology·G A Gerencser, J Zhang
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·G A Gerencser, J Zhang
Jan 13, 2000·Acta Physiologica Scandinavica·S TheanderW Grampp
Jun 14, 2003·Biological Reviews of the Cambridge Philosophical Society·George A Gerencser, Jianliang Zhang
Jan 20, 2004·Biochimica Et Biophysica Acta·George A Gerencser, Jianliang Zhang
Dec 22, 1999·The Journal of Experimental Zoology·G A Gerencser
Mar 10, 2001·The Journal of Experimental Zoology·G A Gerencser, J Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
George A Gerencser, B Zelezna
Biochimica Et Biophysica Acta
George A Gerencser
The Journal of Experimental Zoology
George A Gerencser, J Zhang
© 2021 Meta ULC. All rights reserved