Abstract
Cl- absorption by the Aplysia californica foregut is effected through an active Cl- transport mechanism located in the basolateral membrane of the epithelial absorptive cells. These basolateral membranes contain both Cl(-)-stimulated ATPase and ATP-dependent Cl- transport activities which can be incorporated into liposomes via reconstitution. Utilizing the proteoliposomal preparation, it was demonstrated that ATP, and its subsequent hydrolysis, Mg2+, Cl-, and a pH optimum of 7.8 were required to generate maximal intraliposomal Cl- accumulation, electrical negativity, and ATPase activity. Additionally, an inwardly-directed valinomycin-induced K+ diffusion potential, making the liposome interior electrically positive, enhanced both ATP-driven Cl- accumulation and electrical potential while an outwardly-directed valinomycin-induced K+ diffusion potential, making the liposome interior electrically negative, decreased both ATP-driven Cl- accumulation and electrical potential compared with proteoliposomes lacking the ionophore. Either orthovanadate or p-chloromercurobenzene sulfonate inhibited both the ATP-dependent intraliposomal Cl- accumulation, intraliposomal negative potential difference, and also Cl(-)-stimulated ATPase activit...Continue Reading
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Citations
Jul 13, 2002·The Journal of Experimental Zoology·Georpe A Gerencser, Jianliang Zhang
May 15, 2001·The Journal of Experimental Zoology·G A Gerencser, J Zhang
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·G A Gerencser, J Zhang
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Jun 14, 2003·Biological Reviews of the Cambridge Philosophical Society·George A Gerencser, Jianliang Zhang
Jan 20, 2004·Biochimica Et Biophysica Acta·George A Gerencser, Jianliang Zhang
Dec 22, 1999·The Journal of Experimental Zoology·G A Gerencser
Mar 10, 2001·The Journal of Experimental Zoology·G A Gerencser, J Zhang