Reconstituted human oral and esophageal mucosa in culture

In Vitro Cellular & Developmental Biology. Animal
D OdaS P Lee

Abstract

We have successfully established monolayer and organotypic culture techniques for growing human oral and esophageal epithelial cells. Cells in monolayer culture were grown in serum-free medium, modified from techniques previously reported by our group. The organotypic cultures were grown in a defined medium supplemented with 10% fetal calf serum. Oral and esophageal cells were maintained in keratinocyte basal medium with pituitary extract and other supplements, and 0.05 mM calcium for 7-9 and 9-11 passages, respectively. Both cell types had similar morphology by phase contrast microscopy. When confluent, the cells were predominantly small, basaloid, and uniform and interspersed with larger, differentiated cells. By immunohistochemistry, both cell types in monolayer were positive to AE1, AE3, and 34BE12 antibodies to keratins of stratified epithelia. Oral epithelial cells in monolayer also were positive to 35BH11, representative of simple epithelial keratins, while esophageal cells were not. The esophageal cells were focally positive to K13, while the oral cells were negative. Both were negative for K19. When comparing monolayer to organotypic cultures and to in vivo specimens, there was a significant difference in the expressio...Continue Reading

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Citations

Mar 12, 2009·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Amulya K SaxenaMicheal E Höllwarth
May 26, 2005·Biomaterials·Benjamin L BecksteadCecilia M Giachelli
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Apr 22, 2009·Journal of Experimental Botany·Nick W AlbertKevin M Davies
Dec 3, 2008·Disease Models & Mechanisms·Massimiliano di PietroRebecca C Fitzgerald
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Dec 20, 2007·Oral and Maxillofacial Surgery Clinics of North America·Kenji Izumi, Stephen E Feinberg
Jun 24, 2017·Implant Dentistry·Yiping TongJianfeng Ma
Mar 9, 2005·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Daniela Elena CosteaAnne Christine Johannessen
Apr 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiri KalabisMeenhard Herlyn
May 31, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Lanlan JiangTiantian Wu
Sep 12, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Roxana SchwabWalburgis Brenner
Feb 1, 2003·European Journal of Oral Sciences·Annette HanssonRoland C Grafström
Jul 9, 2008·Scandinavian Journal of Gastroenterology·Daniel CroaghWayne A Phillips

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