Reconstitution and characterization of antibody repertoires of HIV-1-infected "elite neutralizers"

Antiviral Research
Zehua SunMei-Yun Zhang

Abstract

Around 3-5% HIV-1-infected individuals develop high titers of broadly neutralizing HIV-1 antibodies (bnAbs) during chronic infection. However, monoclonal antibodies (mAbs) isolated from such "elite neutralizers" do not, in most cases, depict the serum IgGs in neutralizing the virus. We hypothesize that HIV-1-specific antibodies in infected subjects may work in a population manner in containing the virus in vivo, and in vitro reconstituted antibody repertoires of "elite neutralizers" may mimic the sera in binding and neutralizing the virus. This study aims to investigate the antibody repertoires of three such "elite neutralizers" by reconstituting the immune antibody repertories in vitro followed by a comparative study of the recombinant library IgGs with the corresponding serum IgGs. We found that the recombinant library IgGs were much weaker than the serum IgGs in binding to envelope glycoproteins (Envs) and in neutralizing the virus and inhibiting Env-mediated cell-cell fusion. However, the sorted libraries composing of HIV-1-specific neutralizing antibodies (nAbs) in the three recombinant libraries exhibited comparable binding and inhibitory activities, as well as antibody-dependent cell-mediated cytotoxicity (ADCC), to the ...Continue Reading

References

Dec 1, 1994·AIDS Research and Human Retroviruses·M PurtscherA Jungbauer
Aug 28, 2007·Nature Medicine·Yuxing LiJohn R Mascola
Jun 4, 2010·Expert Review of Vaccines·Liubov ZaritskayaAnatoli M Malyguine
Feb 18, 2014·Cell·Anthony P WestMichel C Nussenzweig
Apr 29, 2014·Cell Reports·Louise ScharfPamela J Bjorkman

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