Reconstitution and protein composition analysis of endocytic actin patches

Current Biology : CB
Alphée MichelotDavid G Drubin


Clathrin-actin-mediated endocytosis in yeast involves the progressive assembly of at least 60 different proteins at cortical sites. More than half of these proteins are involved in the assembly of a branched network of actin filaments to provide the forces required for plasma membrane invagination. To gain insights into the regulation of endocytic actin patch dynamics, we developed an in vitro actin assembly assay using microbeads functionalized with the nucleation promoting factor (NPF) Las17 (yeast WASP). When incubated in a yeast extract, these beads assembled actin networks, and a significant fraction became motile. Multidimensional protein identification technology (MudPIT) showed that the recruitment of actin-binding proteins to these Las17-derived actin networks is selective. None of the proteins known to exclusively regulate the in vivo formation of actin cables or the actin contractile ring were identified. Our analysis also identified components of three other cortical structures, eisosomes, phosphoinositide kinase (PIK) patches, and the TORC2 complex, establishing intriguing biochemical connections between four different yeast cortical complexes. Finally, we identified Aim3 as a regulator of actin dynamics at endocyt...Continue Reading


Apr 1, 1994·The Journal of Cell Biology·J MulhollandD Botstein
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·L A CameronJ A Theriot
May 2, 2001·The Journal of Cell Biology·Bruce L GoodeDavid G Drubin
Aug 30, 2001·Current Biology : CB·Laurent BlanchoinS E Hitchcock-DeGregori
Aug 29, 2002·Nature Cell Biology·Christien J MerrifieldWolfhard Almers
Jun 20, 2003·Current Biology : CB·A A RodalDavid G Drubin
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Asa E Y Engqvist-Goldstein, David G Drubin
Feb 24, 2006·The Journal of Biological Chemistry·Beáta BugyiMiklós Nyitrai
Feb 24, 2006·Nature·Tobias C WaltherPeter Walter
May 26, 2006·Nature Reviews. Molecular Cell Biology·Marko KaksonenDavid G Drubin
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·James B Moseley, Bruce L Goode
Jun 20, 2007·The Journal of Cell Biology·Todd R FlowerStephan N Witt
Mar 19, 2008·The Journal of Cell Biology·Fatima-Zahra IdrissiMaría-Isabel Geli
May 21, 2008·Biochemistry·Rachel E Mahaffy, Thomas D Pollard
Dec 10, 2008·The Journal of Cell Biology·Guido GrossmannWidmar Tanner
Dec 17, 2008·The Journal of Cell Biology·Dan BairdScott D Emr
Jan 16, 2009·Molecular Biology of the Cell·Doris Berchtold, Tobias C Walther
Feb 27, 2009·Molecular Biology of the Cell·Colleen T SkauDavid R Kovar
Oct 31, 2009·Trends in Biochemical Sciences·Nadine Cybulski, Michael N Hall
Jan 12, 2010·Developmental Cell·Shawn M FergusonPietro De Camilli
Jan 23, 2010·Science·Michael CostanzoCharles Boone
Mar 2, 2010·Current Biology : CB·Vérane AchardRajaa Boujemaa-Paterski
Jun 12, 2010·Current Opinion in Cell Biology·Elizabeth Conibear
Jul 1, 2010·Molecular Biology of the Cell·Vladimir SirotkinThomas D Pollard
Aug 14, 2010·Current Biology : CB·Joseph E ClaytonMatthew Lord


Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takuma KishimotoDavid G Drubin
Oct 17, 2012·The Journal of Biological Chemistry·Minjung ChaeGeorge M Carman
Jan 22, 2013·The Journal of Biological Chemistry·Minjung Chae, George M Carman
Sep 14, 2012·Molecular Biology of the Cell·Daniel Feliciano, Santiago M Di Pietro
Oct 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yansong MiaoDavid G Drubin
Jul 28, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Tiffany N CazaAndras Perl
Jan 17, 2012·Trends in Cell Biology·Natasza E ZiółkowskaTobias C Walther
Oct 18, 2011·European Journal of Cell Biology·Joanna KaminskaTeresa Zoladek
Oct 25, 2011·Trends in Cell Biology·Jasper S Weinberg, David G Drubin
Sep 18, 2015·Molecular Biology of the Cell·Xuanli YaoXin Xiang
Jul 28, 2011·The EMBO Journal·Cornelia Kilchert, Anne Spang
May 15, 2015·Molecular Biology of the Cell·Yutian PengGeorjana Barnes
Apr 29, 2015·Current Biology : CB·Laurène GressinAlphée Michelot
Apr 22, 2014·Current Biology : CB·Jasper S Weinberg, David G Drubin
Jan 22, 2013·Developmental Cell·Alphée MichelotDavid G Drubin
Jul 26, 2011·Current Biology : CB·Alphée Michelot, David G Drubin
May 26, 2016·Biophysical Journal·Antoine Jégou, Guillaume Romet-Lemonne
Apr 17, 2016·Journal of Cell Science·Rebecca LuYidi Sun
Mar 16, 2018·G3 : Genes - Genomes - Genetics·Kristie WrasmanBeverly Wendland
Aug 1, 2019·Biology Open·Oliver GlombNils Johnsson
Jan 20, 2019·The Journal of Cell Biology·Ross T A Pedersen, David G Drubin
Aug 9, 2019·Journal of Cell Science·Cameron Dale MacQuarrieVladimir Sirotkin
Feb 7, 2015·Genetics·Bruce L GoodeBeverly Wendland
Oct 7, 2020·Annual Review of Cell and Developmental Biology·Rachel S KadzikDavid R Kovar
Aug 30, 2019·Current Opinion in Genetics & Development·Katrina B Velle, Lillian K Fritz-Laylin

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