Reconstitution fluid type does not affect pulmonary inflammation or DNA damage following infusion of lyophilized plasma

The Journal of Trauma and Acute Care Surgery
Sean P McCullyMartin A Schreiber

Abstract

Dysfunctional inflammation following traumatic hemorrhage can lead to multiple-organ failure and death. In our polytrauma swine model, lyophilized plasma (LP) reconstituted with sterile water and ascorbic acid suppressed systemic inflammation and attenuated DNA damage. However, it remains unknown whether the inflammatory response is affected by the type of fluid used to reconstitute LP. We hypothesized that common resuscitation fluids such as normal saline (LP-NS), lactated Ringer's solution (LP-LR), Hextend (LP-HX), or sterile water (LP-SW) would yield similar inflammation profiles and DNA damage following LP reconstitution and transfusion. This was a randomized, prospective, blinded animal study. LP was reconstituted to 50% of original volume with NS, LR, HX, or SW buffered with 15-mM ascorbic acid. Forty swine were subjected to a validated model of polytrauma, hemorrhagic shock, and Grade V liver injury and resuscitated with LP. Serum interleukin 6 (IL-6), IL-10, plasma C-reactive protein, and 8-hydroxy-2-deoxyguanosine concentrations were assessed for systemic inflammation and DNA damage at baseline, 2 hours, and 4 hours following liver injury. Lung inflammation was evaluated by Real Time Polymerize Chain Reaction (RT-PCR)....Continue Reading

References

Mar 18, 2000·Archives of Surgery·F GebhardU B Brückner
Jun 19, 2003·The Journal of Trauma·Karim BrohiTimothy Coats
Feb 8, 2006·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·N Ni Choileain, H P Redmond
Dec 1, 2007·Injury·Andreas LenzWilliam G Cheadle
May 13, 2008·The Journal of Trauma·Karim BrohiJean-François Pittet
Oct 3, 2009·Archives of Surgery·Nicholas SpoerkeMartin Schreiber
Feb 18, 2010·Shock·Heena P Santry, Hasan B Alam
Mar 28, 2012·The British Journal of Surgery·J MansonK Brohi
Sep 27, 2012·Current Opinion in Critical Care·Daniel Frith, Karim Brohi
Jan 18, 2013·Transfusion·Tim H LeeMartin A Schreiber
Apr 16, 2013·Transfusion·Jürgen BuxEdgar Scheel
Aug 10, 2013·The Journal of Trauma and Acute Care Surgery·Tim H LeeMartin A Schreiber

❮ Previous
Next ❯

Citations

Dec 29, 2017·The Journal of Trauma and Acute Care Surgery·Sawyer SmithMartin Schreiber
Jan 29, 2021·The Journal of Trauma and Acute Care Surgery·Garrick MokLuis Teodoro da Luz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.