Jan 1, 1989

Reconstitution in vitro of human gingiva

Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie
F GosselinM M Portier

Abstract

A model of human gingiva to be used in pharmacological, basic and clinical research was performed in vitro. This model was obtained through a method of low density seeding epithelialization, from a seeding made up of dissociated human gingiva epithelial cells, of a connective tissue equivalent composed of human fibroblasts included in a collagen gel. The histological and ultrastructural data show a multilayered epithelium and the biochemical analysis (two dimensional gel electrophoresis known as NEPHGE) of the cytokeratins used as molecular markers for epithelial differentiation shows the precise differentiation state of the epithelium thus reconstituted. Even though this model has less of a differentiation than that of an in vivo gingival epithelium, it does actually reproduce exactly the structures of the human gingiva namely a multilayered epithelium lying on a connective tissue. It also offers the advantage of cellular elements which are compatible with gingival graftings.

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Mentioned in this Paper

Interdental Papilla
Epithelial Cells
2D Polyacrylamide Gel Electrophoresis
Epidermis
Keratin-Associated Proteins

About this Paper

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