PMID: 9435270Jan 22, 1998Paper

Reconstitution of a regulated transepithelial water pathway in cells transfected with AQP2 and an AQP1/AQP2 hybrid containing the AQP2-C terminus

The Journal of Membrane Biology
R TorianoM Parisi

Abstract

Transepithelial water permeability was measured in LLC-PK1 cells stably transfected with aquaporins (AQPs): AQP1, AQP2, and a chimera of AQP1 and AQP2 containing 41 amino acids of the C-terminus of AQP2. Transepithelial water fluxes (Jw) were not previously reported in cells transfected with aquaporins. Jw were now recorded each minute using a specially developed experimental device. A significant increase in Posm after forskolin (FK) plus vasopressin (VP) was found in AQP2 transfected cells (39.9 +/- 8.2 vs. 12.5 +/- 3.3 cm.sec-1.10(-3)), but not in cells transfected with AQP1 (15.3 +/- 3.6 vs. 13.4 +/- 3.6 cm.sec-1.10(-3)). In the case of the AQP1/2 cells (chimera) the FK plus VP induced Posm was smaller than in AQP2 cells but significantly higher than in mock cells at rest (18.1 +/- 4.8 vs. 6.7 +/- 1.0 cm.sec-1.10(-3)). The increases in Posm values were not paralleled by increases in 14C-Mannitol permeability. HgCl2 inhibited the hydrosmotic response to FK plus VP in AQP2 transfected epithelia. Results were comparable to those observed, in parallel experiments, in a native ADH-sensitive water channel containing epithelial barrier (the toad urinary bladder). Electron microscopy showed confluent LLC-PK1 cells with microvilli a...Continue Reading

Citations

Jul 9, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Claudia SilbersteinCristina Ibarra
Dec 1, 2007·Journal of Biological Physics·Mario ParisiRoxana Toriano
Jun 22, 2005·Journal of Biochemical and Biophysical Methods·Marcelo OzuMario Parisi
Sep 19, 2008·American Journal of Physiology. Cell Physiology·Paula NunesDennis Brown
May 20, 1999·The Journal of Physiology·T Ma, A S Verkman
Mar 29, 2002·The Journal of Investigative Dermatology·Rachid SougratJean-Marc Verbavatz
Dec 20, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Roxana TorianoClaudia Capurro
Jan 25, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M OzuM Parisi
Aug 19, 2005·American Journal of Physiology. Cell Physiology·Cathy Le MoellicNicolette Farman
Aug 16, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Albert MennoneJames L Boyer
Nov 2, 2004·The Journal of Biological Chemistry·Teresa M Buck, William R Skach
Aug 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·R TorianoM Parisi
Jan 17, 2014·American Journal of Physiology. Renal Physiology·Wararat KittikulsuthDonald E Kohan
Nov 20, 1998·The American Journal of the Medical Sciences·M A Baum, H W Harris

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