Reconstitution of composite actin and keratin networks in vesicles

Soft Matter
J DeekAndreas R Bausch

Abstract

Although cytoskeletal networks are interpenetrating and interacting in living cells, very little is understood as to the effect their interaction has on their properties. Here, as a step towards elucidating the synergistic cellular role of these structural proteins, we investigate isolated keratin and actin composites and show how the in vitro network formation of keratin influences the properties of actin networks and vice versa. By encapsulating purified composite networks into vesicles and separating the time scales of network formation we are able to demonstrate that the actin network stabilizes keratin networks by providing an elastic resistance to their collapse in vitro.

References

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Citations

Oct 18, 2019·Soft Matter·Yashar Bashirzadeh, Allen P Liu
Jul 25, 2019·Scientific Reports·Takayasu KawasakiAkinori Irizawa
Jul 11, 2020·Advanced Biosystems·Daniel HürtgenPetra Schwille
May 1, 2021·Nature Reviews. Molecular Cell Biology·Hui Yi Grace Lim, Nicolas Plachta
May 7, 2021·Journal of Proteomics·Yan WangGuangtao Yan
May 15, 2021·ACS Synthetic Biology·Jessica G BermudezMatthew C Good
Jun 30, 2021·ACS Synthetic Biology·Lori Van de CauterKristina A Ganzinger
Aug 28, 2021·Cells·Emma J van Bodegraven, Sandrine Etienne-Manneville

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Methods Mentioned

BETA
confocal microscopy
fluorescence assay
fluorescence microscopy
fluorescence imaging

Software Mentioned

Imaris
cDICE

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