PMID: 6293877Nov 1, 1982Paper

Reconstitution of cytochrome bc1 complex into lipid vesicles and the restoration of uncoupler sensitivity

FEBS Letters
P R Rich, S D Clarke

Abstract

Beef heart mitochondrial bc1 complex (ubiquinone--cytochrome c oxidoreductase) has been assayed by its ability to catalyse the reaction of duroquinol reduction of ferricytochrome c. When the isolated complex is reincorporated into lipid vesicles, the enzyme-catalysed electron transfer rate becomes uncoupler-sensitive. Initial experiments suggest that a protonmotive force is generated across the vesicles when electron transfer is initiated. Both delta psi and delta pH components of this protonmotive force then influence an internal rate constant of the bc1 complex.

References

Oct 21, 1976·Journal of Theoretical Biology·P Mitchell
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G B WarrenJ C Metcalfe
Oct 17, 1968·European Journal of Biochemistry·J B JacksonL V von Stedingk
Jan 1, 1981·Annual Review of Biochemistry·M WikströmM Saraste
Jan 1, 1982·Faraday Discussions of the Chemical Society·P R Rich

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