Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin

The Journal of Cell Biology
Wenjing LiGohta Goshima


Dynamic microtubules (MTs) are essential for various intracellular events, such as mitosis. In Drosophila melanogaster S2 cells, three MT tip-localizing proteins, Msps/XMAP215, EB1, and Sentin (an EB1 cargo protein), have been identified as being critical for accelerating MT growth and promoting catastrophe events, thus resulting in the formation of dynamic MTs. However, the molecular activity of each protein and the basis of the modulation of MT dynamics by these three factors are unknown. In this paper, we showed in vitro that XMAP215(msps) had a potent growth-promoting activity at a wide range of tubulin concentrations, whereas Sentin, when recruited by EB1 to the growing MT tip, accelerated growth and also increased catastrophe frequency. When all three factors were combined, the growth rate was synergistically enhanced, and rescue events were observed most frequently, but frequent catastrophes restrained the lengthening of the MTs. We propose that MT dynamics are promoted by the independent as well as the cooperative action of XMAP215(msps) polymerase and the EB1-Sentin duo.


May 15, 2013·Nature Cell Biology·Marija ZanicJonathon Howard
Jul 23, 2014·The Journal of Cell Biology·Gohta Goshima, Caitlin Sedwick
Dec 17, 2015·The Journal of Cell Biology·A Agata GłuszekHiroyuki Ohkura
Dec 1, 2015·Trends in Cell Biology·Hugo Bowne-AndersonJonathon Howard
Jun 20, 2014·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Kamlesh K GuptaHolly V Goodson
Nov 13, 2015·Nature Reviews. Molecular Cell Biology·Anna Akhmanova, Michel O Steinmetz
Feb 11, 2014·Current Biology : CB·Sebastian P MaurerThomas Surrey
Jun 28, 2016·Genesis : the Journal of Genetics and Development·Takamasa MizoguchiMotoyuki Itoh
Aug 18, 2016·Brain Research Bulletin·André VoelzmannAndreas Prokop
Nov 2, 2016·The Journal of Cell Biology·Takashi Moriwaki, Gohta Goshima
Nov 9, 2016·The Journal of Cell Biology·Kevin C Slep
Apr 14, 2017·ELife·Janina BaumbachSimon L Bullock
Oct 4, 2017·The Journal of Cell Biology·Babet van der VaartStefan Westermann
Sep 7, 2018·Journal of Cell Science·Zane J BergmanGeorjana Barnes
Feb 8, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jeffrey van Haren, Torsten Wittmann
Aug 24, 2017·Nature Reviews. Molecular Cell Biology·Johanna Roostalu, Thomas Surrey
Jul 20, 2019·PloS One·Jonathan B Leano, Kevin C Slep
May 10, 2015·The Journal of Biological Chemistry·Ray Alfaro-Aco, Sabine Petry
May 18, 2017·The Journal of Cell Biology·Amy E Byrnes, Kevin C Slep
Dec 13, 2018·The Journal of Cell Biology·Tomoya Edzuka, Gohta Goshima
May 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Göker ArpağMarija Zanic
Mar 28, 2018·AIMS Genetics·Yekaterina ZvorykinaIvan Vorobjev


Jan 1, 1997·Annual Review of Cell and Developmental Biology·A Desai, T J Mitchison
Sep 6, 2002·The Journal of Cell Biology·Stephen L RogersRonald D Vale
Jun 17, 2005·Molecular Biology of the Cell·Gohta Goshima, Ronald D Vale
Nov 24, 2005·Current Biology : CB·Gohta GoshimaRonald D Vale
Jun 27, 2006·Nature·Jacob W J KerssemakersMarileen Dogterom
Dec 23, 2006·Current Opinion in Cell Biology·Jonathon Howard, Anthony A Hyman
Apr 7, 2007·Science·Gohta GoshimaNico Stuurman
Dec 7, 2007·Nature·Peter BielingThomas Surrey
Jan 15, 2008·Cell·Gary J BrouhardAnthony A Hyman
Mar 7, 2008·Nature Reviews. Molecular Cell Biology·Anna Akhmanova, Michel O Steinmetz
Mar 28, 2008·Nature Cell Biology·Benjamin VitreIsabelle Arnal
Dec 24, 2008·The Journal of Cell Biology·Peter BielingThomas Surrey
Jan 8, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ram DixitErika L F Holzbaur
Mar 4, 2009·The Journal of Cell Biology·Yulia KomarovaAnna Akhmanova
May 29, 2009·Methods in Molecular Biology·Mónica Bettencourt-Dias, Gohta Goshima
Sep 26, 2009·The Journal of Biological Chemistry·Zhiqing C ZhuHolly V Goodson
Oct 24, 2009·PloS One·Marija ZanicJonathon Howard
Dec 5, 2009·Current Opinion in Cell Biology·Kevin C Slep
May 15, 2010·Methods in Cell Biology·Christopher GellJonathon Howard
Aug 20, 2010·Methods in Cell Biology·Gohta Goshima
Sep 21, 2010·Current Biology : CB·Susana Montenegro GouveiaAnna Akhmanova
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sebastian P MaurerThomas Surrey
Jun 8, 2011·The Journal of Cell Biology·Wenjing LiGohta Goshima
Jun 8, 2011·The Journal of Cell Biology·Babet van der VaartAnna Akhmanova

Related Concepts

Microtubule-Associated Protein 3
Drosophila melanogaster Proteins
CKAP5 gene
EB1 microtubule binding proteins

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.