PMID: 2125006Dec 10, 1990Paper

Reconstitution of electron transport in photosystem I with PsaC and PsaD proteins expressed in Escherichia coli

FEBS Letters
J D ZhaoJ H Golbeck

Abstract

A fusion protein, denoted PsaC1, which contains an amino-terminal extension of five amino acids (MEHSM...) and is derived from an in vitro modified form of the psaC gene of Synechococcus sp. PCC 7002, has been over-expressed in Escherichia coli. The product of the psaD gene of Nostoc sp. PCC 8009 has similarly been over-expressed. The PsaC1 and PsaD proteins can be combined with the photosystem I core protein of Synechococcus sp. PCC 6301 to reconstitute electron transport from P700 to the terminal FA/FB acceptors. Reconstitution was found to be absolutely dependent on reinsertion of the iron-sulfur clusters in the PsaC1 apoprotein and on the presence of the PsaD protein. This implies that the PsaC1 holoprotein does not bind solely to the PsaA/PsaB heterodimer but rather that its interaction with these proteins is mediated through the PsaD protein.

References

Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Feb 28, 1989·Biochimica Et Biophysica Acta·K G ParrettJ H Golbeck
Jun 1, 1989·Plant Molecular Biology·H Böhme, R Haselkorn

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Citations

Apr 1, 1993·Photosynthesis Research·S M RoddayJ Biggins
May 1, 1995·Photosynthesis Research·P R ChitnisR Nechushtai
Nov 1, 1995·Photosynthesis Research·H MatsubaraY Fujita
Aug 18, 2000·Biochimie·Y Choquet, O Vallon
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Carolyn E LubnerJohn H Golbeck
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M MannanH B Pakrasi
Nov 12, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Rebecca Ann GrimmeJohn Harvey Golbeck
Jul 1, 1994·The Journal of Experimental Zoology·T S ArmbrustJ A Guikema
Dec 2, 2009·Biochemistry·Carolyn E LubnerJohn H Golbeck
Oct 8, 1998·Journal of Molecular Biology·D FotiadisA Engel
Nov 1, 2001·Biochimica Et Biophysica Acta·I R VassilievJ H Golbeck
Jul 5, 1993·Biochimica Et Biophysica Acta·N Weber, H Strotmann
Nov 10, 2010·Biochemistry·Carolyn E LubnerJohn H Golbeck

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