Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

Chemical Communications : Chem Comm
Keita OmuraYoshihito Watanabe

Abstract

Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

References

Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M H GelbS G Sligar
Jul 1, 1996·Protein Science : a Publication of the Protein Society·S Govindaraj, T L Poulos
Jun 13, 2001·Molecular Microbiology·S K MazmanianO Schneewind
Oct 1, 1959·Biochimica Et Biophysica Acta·F W TEALE
Jul 26, 2005·Infection and Immunity·Steven D Zink, Drusilla L Burns
Nov 21, 2007·Bioorganic & Medicinal Chemistry Letters·Bala Chandra ChennaSadanandan E Velu
Jan 24, 2009·Angewandte Chemie·Ljiljana FrukChristof M Niemeyer
Dec 22, 2010·Journal of the American Chemical Society·Victor S LelyveldAlan Jasanoff
Mar 18, 2011·Current Opinion in Biotechnology·Sang Taek JungFrances H Arnold
Jun 24, 2011·Proceedings of the National Academy of Sciences of the United States of America·Irwin ChenDavid R Liu
Oct 20, 2011·Chemical Society Reviews·Christopher J C WhitehouseLuet-Lok Wong
Aug 2, 2012·Chembiochem : a European Journal of Chemical Biology·Norifumi KawakamiYoshihito Watanabe
Mar 26, 2016·Current Opinion in Chemical Biology·Hazel M Girvan, Andrew W Munro
Aug 31, 2016·Journal of the American Chemical Society·Evan W ReynoldsEric M Brustad
Mar 13, 2018·Journal of Inorganic Biochemistry·Shaghayegh DezvareiStephen G Bell

❮ Previous
Next ❯

Citations

Jul 11, 2020·Chemistry : a European Journal·Zhi ZouUlrich Schwaneberg
Dec 19, 2019·Frontiers in Chemistry·Timo NuijensWalter Cabri
Sep 26, 2020·Chembiochem : a European Journal of Chemical Biology·Rodrigo F Ortiz-MeozGuofeng Zhang
Dec 31, 2020·Biotechnology and Applied Biochemistry·Mallory KatoLionel Cheruzel
Aug 12, 2020·Current Opinion in Chemical Biology·Shinya AriyasuOsami Shoji

❮ Previous
Next ❯

Methods Mentioned

BETA
gel-filtration
X-ray

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.